Mouse Anti-NR0B2 Recombinant Antibody (1A11) (CBMAB-N3324-WJ)

Name: Mouse Anti-NR0B2 Recombinant Antibody (1A11)
SKU: CBMAB-N3324-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1A11

Application

ELISA, IF, WB

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

nuclear receptor subfamily 0, group B, member 2

Introduction

The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. [provided by RefSeq, Jul 2008]

Entrez Gene ID

8431

UniProt ID

Q15466

Alternative Names

Nuclear Receptor Subfamily 0 Group B Member 2; Orphan Nuclear Receptor SHP; Small Heterodimer Partner; SHP; Nuclear Receptor Subfamily 0, Group B, Member 2; Nuclear Receptor SHP; SHP1;

Function

Transcriptional regulator that acts as a negative regulator of receptor-dependent signaling pathways (By similarity).
Specifically inhibits transactivation of the nuclear receptor with which it interacts (By similarity).
Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated transcription complex for NEUROD1 (PubMed:14752053).
Essential component of the liver circadian clock which via its interaction with NR1D1 and RORG regulates NPAS2-mediated hepatic lipid metabolism (By similarity).
Regulates the circadian expression of cytochrome P450 (CYP) enzymes (By similarity).
Represses: NR5A2 and HNF4A to down-regulate CYP2C38, NFLI3 to up-regulate CYP2A5, BHLHE41/HNF1A axis to up-regulate CYP1A2, CYP2E1 and CYP3A11, and NR1D1 to up-regulate CYP2B10, CYP4A10 and CYP4A14 (By similarity).

Biological Process

Animal organ regenerationIEA:Ensembl
Bile acid and bile salt transportIEA:Ensembl
Cholesterol metabolic processManual Assertion Based On ExperimentTAS:ProtInc
Circadian regulation of gene expressionISS:UniProtKB
Circadian rhythmManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of DNA-binding transcription factor activityManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of gene expressionManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of transcription, DNA-templatedISS:UniProtKB
Notch signaling pathwayIEA:Ensembl
Positive regulation of gene expressionManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of insulin secretionIEA:Ensembl
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:MGI
Response to ethanolIEA:Ensembl
Response to glucoseIEA:Ensembl
Response to organic cyclic compoundIEA:Ensembl

Cellular Location

Nucleus
Cytoplasm
Colocalizes with NEUROD1 in the nucleus.

Involvement in disease

Obesity (OBESITY):
A condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.

PTM

Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.

Vidana Gamage, H. E., Shahoei, S. H., Albright, S., Nguyen, T., Farmer, R. E., Weisser, E., ... & Nelson, E. R. (2023). OR05-01 Myeloid Cell Intrinsic NR0B2 Inhibits Regulatory T Cell Expansion Within The Tumor Microenvironment: Potential Novel Strategy For Breast Cancer Immunotherapy. Journal of the Endocrine Society, 7(Supplement_1), bvad114-2187.

Seok, S., & Kemper, J. K. (2023). Paradoxical feeding activation of gut lipophagy by FGF15/FGF19-NR0B2/SHP-TFEB. Autophagy, 19(2), 742-743.

Zhou, L. M., Fan, J. H., Xu, M. M., Xiong, M. Y., Wang, Q. J., Chai, X., ... & Ye, X. L. (2023). Epiberberine regulates lipid synthesis through SHP (NR0B2) to improve non-alcoholic steatohepatitis. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1869(4), 166639.

Desterke, C., Chung, C., Pan, D., Trauner, M., Samuel, D., Azoulay, D., & Feray, C. (2023). Early Deregulation of Cholangiocyte NR0B2 During Primary Sclerosing Cholangitis. Gastro Hep Advances, 2(1), 49-62.

Lee, H. I., Kwon, S. S., Lee, M., Kim, S. J., Song, K., Kwon, A., ... & Suh, J. (2023). Maturity-onset diabetes of the young due to NR0B2 gene mutation. Annals of Pediatric Endocrinology & Metabolism, 28(Suppl 1), S3.

Gamage, H. E. V., Shahoei, S. H., Nguyen, T., Farmer, R., Albright, S., Weisser, E., ... & Nelson, E. R. (2023). NR0B2 re-educates myeloid cells within the tumor microenvironment: Potential novel strategy for breast cancer immunotherapy. Cancer Research, 83(7_Supplement), 2358-2358.

Zhu, R., Tu, Y., Chang, J., Xu, H., Li, J. C., Liu, W., ... & Li, B. (2021). The orphan nuclear receptor gene NR0B2 is a favorite prognosis factor modulated by multiple cellular signal pathways in human liver cancers. Frontiers in Oncology, 11, 691199.

Lam, K. K., Sethi, R., Tan, G., Tomar, S., Lo, M., Loi, C., ... & Cheah, P. Y. (2021). The orphan nuclear receptor NR0B2 could be a novel susceptibility locus associated with microsatellite‐stable, APC mutation‐negative early‐onset colorectal carcinomas with metabolic manifestation. Genes, Chromosomes and Cancer, 60(2), 61-72.

Desterke, C., & Feray, C. (2021). NR0B2 regulation during Primary Sclerosing Cholangitis defines a metabolic and pre-malignant reprogramming of Cholangiocyte. bioRxiv, 2021-09.

Tu, Y., Li, J., & Zhang, L. (2020). Abstract B34: PI3K/p110β modulates tumor suppressor gene NR0B2 expression in human liver cancer cells. Molecular Cancer Research, 18(10_Supplement), B34-B34.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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