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Mouse Anti-NR1I3 Recombinant Antibody (N4111) (CBMAB-C12149-LY)

The product is antibody recognizes NR1I3. The antibody N4111 immunoassay techniques such as: WB, IHC, IP.
See all NR1I3 antibodies
Published Data
Fig.1 The resulting total precipitated ubiquitinated protein was subjected to Western blot detection with an anti-hCAR antibody.
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Fig.2 Illustrative images of CAR staining in liver sections from a healthy control and a fasted critically ill animal.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
N4111
Antibody Isotype
IgG1
Application
WB, IHC, IP

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Nuclear Receptor Subfamily 1 Group I Member 3
Introduction
This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Entrez Gene ID
9970
UniProt ID
Q14994
Alternative Names
Nuclear Receptor Subfamily 1 Group I Member 3; Constitutive Androstane Receptor; Constitutive Activator Of Retinoid Response; Constitutive Active Response; Orphan Nuclear Receptor MB67; CAR; Constitutive Androstane Nuclear Receptor Variant 2; Constitutive Androstane Nuclear Receptor Variant 3;
Function
Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element.
Biological Process
Cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Osteoblast differentiationIEA:Ensembl
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:NTNU_SB
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Nucleus
Cytoplasm
Cytoplasm, cytoskeleton
Recruited to the cytoplasm by DNAJC7.
PTM
Phosphorylated at Thr-38 by PKC, dephosphorylation of Thr-38 is required for nuclear translocation and activation.

de Almeida Velozo, C., de Almeida, T. B., de Azevedo, M. C. V. M., Espasandin, I., da Cunha Pinto, J. F., López, S., ... & Cardoso, C. C. (2022). Polymorphisms at CYP enzymes, NR1I2 and NR1I3 in association with virologic response to antiretroviral therapy in Brazilian HIV-positive individuals. The Pharmacogenomics Journal, 22(1), 33-38.

Wagner, F., Ruf, I., Lehmann, T., Hofmann, R., Ortmann, S., Schiffmann, C., ... & Stuckas, H. (2022). Reconstruction of evolutionary changes in fat and toxin consumption reveals associations with gene losses in mammals: A case study for the lipase inhibitor PNLIPRP1 and the xenobiotic receptor NR1I3. Journal of evolutionary biology, 35(2), 225-239.

Mazin, M. E., Yarushkin, A. A., Pustylnyak, Y. A., Prokopyeva, E. A., & Pustylnyak, V. O. (2022). Promotion of NR1I3-mediated liver growth is accompanied by STAT3 activation. Molecular Biology Reports, 49(5), 4089-4093.

Pinne, M., & Raucy, J. L. (2021). Cytochrome P450 Gene Regulation: Reporter Assays to Assess Direct and Indirect Activation of Constitutive Androstane Receptor (CAR, NR1I3). Cytochrome P450: In Vitro Methods and Protocols, 175-192.

Deng, Y., Wei, Z., Huang, M., Xu, G., Wei, W., Peng, B., ... & Qin, H. (2020). Long non‐coding RNA F11‐AS1 inhibits HBV‐related hepatocellular carcinoma progression by regulating NR1I3 via binding to microRNA‐211‐5p. Journal of Cellular and Molecular Medicine, 24(2), 1848-1865.

Chen, M. L., Huang, X., Wang, H., Hegner, C., Liu, Y., Shang, J., ... & Sundrud, M. S. (2020). CAR/Nr1i3 directs T cell adaptation to bile acids in the small intestine. bioRxiv, 2020-07.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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