Mouse Anti-NR1I3 Recombinant Antibody (N4111) (CBMAB-C12149-LY)

Name: Mouse Anti-NR1I3 Recombinant Antibody (N4111)
SKU: CBMAB-C12149-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

N4111

Application

WB, IHC, IP

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Nuclear Receptor Subfamily 1 Group I Member 3

Introduction

This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Entrez Gene ID

9970

UniProt ID

Q14994

Alternative Names

Nuclear Receptor Subfamily 1 Group I Member 3; Constitutive Androstane Receptor; Constitutive Activator Of Retinoid Response; Constitutive Active Response; Orphan Nuclear Receptor MB67; CAR; Constitutive Androstane Nuclear Receptor Variant 2; Constitutive Androstane Nuclear Receptor Variant 3;

Function

Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element.

Biological Process

Cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Osteoblast differentiationIEA:Ensembl
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:NTNU_SB
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Nucleus
Cytoplasm
Cytoplasm, cytoskeleton
Recruited to the cytoplasm by DNAJC7.

PTM

Phosphorylated at Thr-38 by PKC, dephosphorylation of Thr-38 is required for nuclear translocation and activation.

de Almeida Velozo, C., de Almeida, T. B., de Azevedo, M. C. V. M., Espasandin, I., da Cunha Pinto, J. F., López, S., ... & Cardoso, C. C. (2022). Polymorphisms at CYP enzymes, NR1I2 and NR1I3 in association with virologic response to antiretroviral therapy in Brazilian HIV-positive individuals. The Pharmacogenomics Journal, 22(1), 33-38.

Wagner, F., Ruf, I., Lehmann, T., Hofmann, R., Ortmann, S., Schiffmann, C., ... & Stuckas, H. (2022). Reconstruction of evolutionary changes in fat and toxin consumption reveals associations with gene losses in mammals: A case study for the lipase inhibitor PNLIPRP1 and the xenobiotic receptor NR1I3. Journal of evolutionary biology, 35(2), 225-239.

Mazin, M. E., Yarushkin, A. A., Pustylnyak, Y. A., Prokopyeva, E. A., & Pustylnyak, V. O. (2022). Promotion of NR1I3-mediated liver growth is accompanied by STAT3 activation. Molecular Biology Reports, 49(5), 4089-4093.

Pinne, M., & Raucy, J. L. (2021). Cytochrome P450 Gene Regulation: Reporter Assays to Assess Direct and Indirect Activation of Constitutive Androstane Receptor (CAR, NR1I3). Cytochrome P450: In Vitro Methods and Protocols, 175-192.

Deng, Y., Wei, Z., Huang, M., Xu, G., Wei, W., Peng, B., ... & Qin, H. (2020). Long non‐coding RNA F11‐AS1 inhibits HBV‐related hepatocellular carcinoma progression by regulating NR1I3 via binding to microRNA‐211‐5p. Journal of Cellular and Molecular Medicine, 24(2), 1848-1865.

Chen, M. L., Huang, X., Wang, H., Hegner, C., Liu, Y., Shang, J., ... & Sundrud, M. S. (2020). CAR/Nr1i3 directs T cell adaptation to bile acids in the small intestine. bioRxiv, 2020-07.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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