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Rabbit Anti-NSUN2 Recombinant Antibody (3H24L11) (CBMAB-N3672-WJ)

This product is a Rabbit antibody that recognizes NSUN2. The antibody 3H24L11 can be used for immunoassay techniques such as: FC, ICC, IF, WB.
See all NSUN2 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
3H24L11
Antibody Isotype
IgG
Application
FC, ICC, IF, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
0.09% sodium azide
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NOP2/Sun RNA Methyltransferase Family Member 2
Introduction
This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011]
Entrez Gene ID
54888
UniProt ID
Q08J23
Alternative Names
NOP2/Sun RNA Methyltransferase Family Member 2; Myc-Induced SUN-Domain-Containing Protein; Myc-Induced SUN Domain-Containing Protein; NOL1/NOP2/Sun Domain Family, Member 2; Substrate Of AIM1/Aurora Kinase B; NOP2/Sun Domain Family, Member 2; TRNA Methyltransferase 4 Homolog; MISU; SAKI; TRM4; Mental Retardation, Non-Syndromic, Autosomal Recessive, 5; TRNA Methyltransferase 4 Homolog (S. Cerevisiae);
Function
RNA cytosine C5-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs) (PubMed:17071714, PubMed:22995836, PubMed:31358969, PubMed:31199786).
Involved in various processes, such as epidermal stem cell differentiation, testis differentiation and maternal to zygotic transition during early development: acts by increasing protein synthesis; cytosine C5-methylation promoting tRNA stability and preventing mRNA decay (PubMed:31199786).
Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors (PubMed:17071714, PubMed:22995836, PubMed:31199786).
tRNA methylation is required generation of RNA fragments derived from tRNAs (tRFs) (PubMed:31199786).
Also mediates C5-methylation of mitochondrial tRNAs (PubMed:31276587).
Catalyzes cytosine C5-methylation of mRNAs, leading to stabilize them and prevent mRNA decay: mRNA stabilization involves YBX1 that specifically recognizes and binds m5C-modified transcripts (PubMed:22395603, PubMed:31358969, PubMed:34556860).
Cytosine C5-methylation of mRNAs also regulates mRNA export: methylated transcripts are specifically recognized by THOC4/ALYREF, which mediates mRNA nucleo-cytoplasmic shuttling (PubMed:28418038).
Also mediates cytosine C5-methylation of non-coding RNAs, such as vault RNAs (vtRNAs), promoting their processing into regulatory small RNAs (PubMed:23871666).
Cytosine C5-methylation of vtRNA VTRNA1.1 promotes its processing into small-vault RNA4 (svRNA4) and regulates epidermal differentiation (PubMed:31186410).
May act downstream of Myc to regulate epidermal cell growth and proliferation (By similarity).
Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity (PubMed:19596847).
Biological Process
Cell divisionIEA:UniProtKB-KW
Hair follicle maturationISS:UniProtKB
In utero embryonic developmentIEA:Ensembl
Meiotic cell cycle checkpoint signalingIEA:Ensembl
mRNA methylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of mRNA export from nucleusManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of stem cell differentiationISS:UniProtKB
RNA methylationManual Assertion Based On ExperimentIBA:GO_Central
Spermatid developmentIEA:Ensembl
tRNA methylationManual Assertion Based On ExperimentIDA:UniProtKB
tRNA modificationTAS:Reactome
tRNA stabilizationISS:UniProtKB
Cellular Location
Nucleus, nucleolus
Cytoplasm
Mitochondrion
Cytoplasm, cytoskeleton, spindle
Secreted, extracellular exosome
Concentrated in the nucleolus during interphase and translocates to the spindle during mitosis as an RNA-protein complex that includes 18S ribosomal RNA (PubMed:19596847).
In testis, localizes to the chromatoid body (By similarity).
Involvement in disease
Mental retardation, autosomal recessive 5 (MRT5):
A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period.
PTM
Phosphorylated at Ser-139 by AURKB during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1.

Lin, L., Deng, C., Zhou, C., Zhang, X., Zhu, J., Liu, J., ... & He, J. (2023). NSUN2 gene rs13181449 C> T polymorphism reduces neuroblastoma risk. Gene, 854, 147120.

Liu, M., Guo, G., Qian, P., Mu, J., Lu, B., He, X., ... & Zhang, Q. (2022). 5-methylcytosine modification by Plasmodium NSUN2 stabilizes mRNA and mediates the development of gametocytes. Proceedings of the National Academy of Sciences, 119(9), e2110713119.

Hu, Y., Chen, C., Tong, X., Chen, S., Hu, X., Pan, B., ... & Lu, M. (2021). NSUN2 modified by SUMO-2/3 promotes gastric cancer progression and regulates mRNA m5C methylation. Cell Death & Disease, 12(9), 842.

Su, J., Wu, G., Ye, Y., Zhang, J., Zeng, L., Huang, X., ... & Zheng, J. (2021). NSUN2-mediated RNA 5-methylcytosine promotes esophageal squamous cell carcinoma progression via LIN28B-dependent GRB2 mRNA stabilization. Oncogene, 40(39), 5814-5828.

Xu, X., Zhang, Y., Zhang, J., & Zhang, X. (2020). NSun2 promotes cell migration through methylating autotaxin mRNA. Journal of Biological Chemistry, 295(52), 18134-18147.

Chellamuthu, A., & Gray, S. G. (2020). The RNA methyltransferase NSUN2 and its potential roles in cancer. Cells, 9(8), 1758.

Mei, L., Shen, C., Miao, R., Wang, J. Z., Cao, M. D., Zhang, Y. S., ... & Wei, J. F. (2020). RNA methyltransferase NSUN2 promotes gastric cancer cell proliferation by repressing p57Kip2 by an m5C-dependent manner. Cell death & disease, 11(4), 270.

Sun, Z., Xue, S., Zhang, M., Xu, H., Hu, X., Chen, S., ... & Cui, H. (2020). Aberrant NSUN2-mediated m5C modification of H19 lncRNA is associated with poor differentiation of hepatocellular carcinoma. Oncogene, 39(45), 6906-6919.

Shinoda, S., Kitagawa, S., Nakagawa, S., Wei, F. Y., Tomizawa, K., Araki, K., ... & Suzuki, T. (2019). Mammalian NSUN2 introduces 5-methylcytidines into mitochondrial tRNAs. Nucleic acids research, 47(16), 8734-8745.

Van Haute, L., Lee, S. Y., McCann, B. J., Powell, C. A., Bansal, D., Vasiliauskaitė, L., ... & Minczuk, M. (2019). NSUN2 introduces 5-methylcytosines in mammalian mitochondrial tRNAs. Nucleic acids research, 47(16), 8720-8733.

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For research use only. Not intended for any clinical use.

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