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Rabbit Anti-NSUN5 Recombinant Antibody (4HCLC) (CBMAB-N3673-WJ)

This product is a Rabbit antibody that recognizes NSUN5. The antibody 4HCLC can be used for immunoassay techniques such as: FC, IF, WB.
See all NSUN5 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
4HCLC
Antibody Isotype
IgG
Application
FC, IF, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
0.09% sodium azide
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NOP2/Sun RNA Methyltransferase Family Member 5
Introduction
This gene encodes a member of an evolutionarily conserved family of proteins that may function as methyltransferases. This gene is located in a larger region of chromosome 7 that is deleted in Williams-Beuren syndrome, a multisystem developmental disorder. There are two pseudogenes for this gene located in the same region of chromosome 7. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
Entrez Gene ID
55695
UniProt ID
Q96P11
Alternative Names
NOP2/Sun RNA Methyltransferase Family Member 5; Williams-Beuren Syndrome Chromosomal Region 20A Protein; Williams Beuren Syndrome Chromosome Region 20A; NOL1/NOP2/Sun Domain Family Member 5; NOP2/Sun Domain Family, Member 5A; NOP2/Sun Domain Family, Member 5; NOL1-Related Protein; WBSCR20A; WBSCR20; NSUN5A;
Function
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 3782 (m5C3782) in 28S rRNA (PubMed:23913415, PubMed:31428936, PubMed:31722427).
m5C3782 promotes protein translation without affecting ribosome biogenesis and fidelity (PubMed:31428936, PubMed:31722427).
Required for corpus callosum and cerebral cortex development (By similarity).
Biological Process
Cerebral cortex developmentISS:UniProtKB
CognitionISS:UniProtKB
Corpus callosum developmentISS:UniProtKB
Oligodendrocyte developmentISS:UniProtKB
Positive regulation of translationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of myelinationISS:UniProtKB
RNA methylationManual Assertion Based On ExperimentIBA:GO_Central
rRNA base methylationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus, nucleolus
Involvement in disease
NSUN5 is located in the Williams-Beuren syndrome (WBS) critical region (PubMed:11978965, PubMed:12073013). WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region (PubMed:11978965, PubMed:12073013). Its deletion in WBS results in decreased methylation of the C(5) position of cytosine 3782 (m5C3782) in 28S rRNA (PubMed:31722427).

Gu, X., Li, P., Gao, X., Ru, Y., Xue, C., Zhang, S., ... & Hu, X. (2024). RNA 5-methylcytosine writer NSUN5 promotes hepatocellular carcinoma cell proliferation via a ZBED3-dependent mechanism. Oncogene, 1-12.

Zhou, J., Kong, Y. S., Vincent, K. M., Dieters‐Castator, D., Bukhari, A. B., Glubrecht, D., ... & Fu, Y. (2023). RNA cytosine methyltransferase NSUN5 promotes protein synthesis and tumorigenic phenotypes in glioblastoma. Molecular Oncology, 17(9), 1763-1783.

Zhang, X. W., Wu, L. Y., Liu, H. R., Huang, Y., Qi, Q., Zhong, R., ... & Wu, H. G. (2022). NSUN5 promotes progression and predicts poor prognosis in hepatocellular carcinoma. Oncology Letters, 24(6), 1-10.

Liu, J., Ren, Z., Yang, L., Zhu, L., Li, Y., Bie, C., ... & Kuang, W. (2022). The NSUN5-FTH1/FTL pathway mediates ferroptosis in bone marrow-derived mesenchymal stem cells. Cell Death Discovery, 8(1), 99.

Sun, B., Zeng, H., Liang, J., Zhang, L., Hu, H., Wang, Q., ... & Zhu, J. (2020). NSUN5 facilitates viral RNA recognition by RIG-I receptor. The Journal of Immunology, 205(12), 3408-3418.

Jiang, Z., Li, S., Han, M. J., Hu, G. M., & Cheng, P. (2020). High expression of NSUN5 promotes cell proliferation via cell cycle regulation in colorectal cancer. American journal of translational research, 12(7), 3858.

Janin, M., Ortiz-Barahona, V., de Moura, M. C., Martínez-Cardús, A., Llinàs-Arias, P., Soler, M., ... & Esteller, M. (2019). Epigenetic loss of RNA-methyltransferase NSUN5 in glioma targets ribosomes to drive a stress adaptive translational program. Acta neuropathologica, 138, 1053-1074.

Heissenberger, C., Liendl, L., Nagelreiter, F., Gonskikh, Y., Yang, G., Stelzer, E. M., ... & Schosserer, M. (2019). Loss of the ribosomal RNA methyltransferase NSUN5 impairs global protein synthesis and normal growth. Nucleic acids research, 47(22), 11807-11825.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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