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Mouse Anti-NT5C2 Recombinant Antibody (3C1) (CBMAB-N3677-WJ)

This product is a Mouse antibody that recognizes NT5C2. The antibody 3C1 can be used for immunoassay techniques such as: ELISA, IF, IHC-P, WB.
See all NT5C2 antibodies
Published Data
Fig.1 Effect of cN-II silencing on protein synthesis in A549 cells.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
3C1
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, IHC-P, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
5'-nucleotidase, cytosolic II
Introduction
This gene encodes a hydrolase that serves as an important role in cellular purine metabolism by acting primarily on inosine 5'-monophosphate and other purine nucleotides. [provided by RefSeq, Oct 2011]
Entrez Gene ID
22978
UniProt ID
P49902
Alternative Names
5'-Nucleotidase, Cytosolic II; 5-Nucleotidase, Cytosolic II; 5-Nucleotidase (Purine), Cytosolic Type B; EC 3.1.3.5; NT5B; PNT5; Cytosolic Purine 5-Nucleotidase; Cytosolic 5-Nucleotidase II; Purine 5 Nucleotidase;
Function
Broad specificity cytosolic 5'-nucleotidase that catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates (PubMed:1659319, PubMed:9371705, PubMed:10092873, PubMed:12907246).
In addition, possesses a phosphotransferase activity by which it can transfer a phosphate from a donor nucleoside monophosphate to an acceptor nucleoside, preferably inosine, deoxyinosine and guanosine (PubMed:1659319, PubMed:9371705).
Has the highest activities for IMP and GMP followed by dIMP, dGMP and XMP (PubMed:1659319, PubMed:9371705, PubMed:10092873, PubMed:12907246).
Could also catalyze the transfer of phosphates from pyrimidine monophosphates but with lower efficiency (PubMed:1659319, PubMed:9371705).
Through these activities regulates the purine nucleoside/nucleotide pools within the cell (PubMed:1659319, PubMed:9371705, PubMed:10092873, PubMed:12907246).
Biological Process
Adenosine metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Allantoin metabolic processManual Assertion Based On ExperimentIDA:MGI
dGMP metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
GMP metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
IMP catabolic processManual Assertion Based On ExperimentIDA:MGI
IMP metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Nucleotide phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm, cytosol
Involvement in disease
Spastic paraplegia 45, autosomal recessive (SPG45):
A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Some SPG45 patients manifest mental retardation, contractures and learning disability.

Aabdien, A., Sichlinger, L., Borgel, Z., Jones, M. R., Waston, I. A., Gatford, N. J., ... & Srivastava, D. P. (2024). Schizophrenia risk proteins ZNF804A and NT5C2 interact in cortical neurons. European Journal of Neuroscience.

Reglero, C., Dieck, C. L., Zask, A., Forouhar, F., Laurent, A. P., Lin, W. H. W., ... & Ferrando, A. A. (2022). Pharmacologic inhibition of NT5C2 reverses genetic and nongenetic drivers of 6-MP resistance in acute lymphoblastic leukemia. Cancer discovery, 12(11), 2646-2665.

Naseer, M. I., Abdulkareem, A. A., & Pushparaj, P. N. (2020). Exome analysis identified novel homozygous splice site donor alteration in NT5C2 gene in a Saudi family associated with spastic diplegia cerebral palsy, developmental delay, and intellectual disability. Frontiers in Genetics, 11, 513205.

Chen, Y. T., Lin, W. D., Liao, W. L., Tsai, Y. C., Liao, J. W., & Tsai, F. J. (2020). NT5C2 methylation regulatory interplay between DNMT1 and insulin receptor in type 2 diabetes. Scientific Reports, 10(1), 16087.

Barz, M. J., Hof, J., Groeneveld-Krentz, S., Loh, J. W., Szymansky, A., Astrahantseff, K., ... & Kirschner-Schwabe, R. (2020). Subclonal NT5C2 mutations are associated with poor outcomes after relapse of pediatric acute lymphoblastic leukemia. Blood, The Journal of the American Society of Hematology, 135(12), 921-933.

Chen, X., Zhang, Z., Wang, X., Chen, Y., & Wang, C. (2020). NT5C2 gene polymorphisms and the risk of coronary heart disease. Public health genomics, 23(3-4), 90-99.

Vishnolia, K. K., Hoene, C., Tarhbalouti, K., Revenstorff, J., Aherrahrou, Z., & Erdmann, J. (2020). Studies in zebrafish demonstrate that CNNM2 and NT5C2 are most likely the causal genes at the blood pressure-associated locus on human chromosome 10q24. 32. Frontiers in Cardiovascular Medicine, 7, 135.

Duarte, R. R., Bachtel, N. D., Côtel, M. C., Lee, S. H., Selvackadunco, S., Watson, I. A., ... & Srivastava, D. P. (2019). The psychiatric risk gene NT5C2 regulates adenosine monophosphate-activated protein kinase signaling and protein translation in human neural progenitor cells. Biological psychiatry, 86(2), 120-130.

Dieck, C. L., & Ferrando, A. (2019). Genetics and mechanisms of NT5C2-driven chemotherapy resistance in relapsed ALL. Blood, The Journal of the American Society of Hematology, 133(21), 2263-2268.

Moriyama, T., Liu, S., Li, J., Meyer, J., Zhao, X., Yang, W., ... & Yang, J. J. (2019). Mechanisms of NT5C2-mediated thiopurine resistance in acute lymphoblastic leukemia. Molecular cancer therapeutics, 18(10), 1887-1895.

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For research use only. Not intended for any clinical use.

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