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Mouse Anti-NUAK1 Recombinant Antibody (12K12) (CBMAB-A4634-YC)

Provided herein is a Mouse monoclonal antibody against Human NUAK Family Kinase 1. The antibody can be used for immunoassay techniques, such as WB.
See all NUAK1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
12K12
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Recombinant corresponding to aa 311-462 from human ARK5 expressed in E. coli.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized from PBS, 5% trehalose
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NUAK Family Kinase 1
Introduction
NUAK1 is a serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. NUAK1 phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. NUAK1 acts a
Entrez Gene ID
Human9891
Mouse77976
Rat299694
UniProt ID
HumanO60285
MouseQ641K5
RatD3ZQN8
Alternative Names
NUAK Family Kinase 1; AMP-Activated Protein Kinase Family Member 5; NUAK Family, SNF1-Like Kinase, 1; AMPK-Related Protein Kinase 5; Omphalocele Kinase 1; EC 2.7.11.1;
Function
Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate in transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (PubMed:25329316).
Biological Process
Cell adhesionIEA:UniProtKB-KW
Cellular response to DNA damage stimulusIEA:UniProtKB-KW
Cellular response to glucose starvationManual Assertion Based On ExperimentIBA:GO_Central
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell adhesionManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell population proliferationManual Assertion Based On ExperimentTAS:UniProtKB
Regulation of cellular senescenceManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of myosin-light-chain-phosphatase activityManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of signal transduction by p53 class mediatorTAS:Reactome
Cellular Location
Nucleus
Cytoplasm
PTM
Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Not dephosphorylated by the myosin PP1 complex when regulating its activity, due to the presence of PPP1R12A, which prevents myosin PP1 from dephosphorylating NUAK1. Phosphorylated by STK38L upon stimulation with IGF1.

Yao, C. Y., Gao, Z. X., Hou, L. L., & Fang, D. (2023). DKK1 promotes NUAK1 transcriptional expression through the activation Akt in hepatocellular carcinoma. Cell Biology International, 47(2), 383-393.

Palma, M., Riffo, E., Farias, A., Coliboro-Dannich, V., Espinoza-Francine, L., Escalona, E., ... & Castro, A. F. (2023). NUAK1 coordinates growth factor-dependent activation of mTORC2 and Akt signaling. Cell & Bioscience, 13(1), 232.

Liu, Y., Wang, R., Huang, R., Tamalunas, A., Waidelich, R., & Hennenberg, M. (2023). Inhibition of growth and contraction in human prostate stromal cells by silencing of NUAK1 and-2, and by the presumed NUAK inhibitors HTH01-015 and WZ4003. Frontiers in Pharmacology, 14, 1105427.

Zhang, T., He, X., Caldwell, L., Goru, S. K., Ulloa Severino, L., Tolosa, M. F., ... & Yuen, D. A. (2022). NUAK1 promotes organ fibrosis via YAP and TGF-β/SMAD signaling. Science Translational Medicine, 14(637), eaaz4028.

Seo, M. S., Jung, K. H., Kim, K., Lee, J. E., Han, B. S., Ko, S., ... & Hong, S. S. (2022). Discovery of a novel NUAK1 inhibitor against pancreatic cancer. Biomedicine & Pharmacotherapy, 152, 113241.

van de Vis, R. A., Moustakas, A., & van der Heide, L. P. (2021). NUAK1 And NUAK2 fine-tune TGF-β signaling. Cancers, 13(13), 3377.

Yang, C., Zhang, Y., Lin, S., Liu, Y., & Li, W. (2021). Suppressing the KIF20A/NUAK1/Nrf2/GPX4 signaling pathway induces ferroptosis and enhances the sensitivity of colorectal cancer to oxaliplatin. Aging (Albany NY), 13(10), 13515.

Escalona, E., Muñoz, M., Pincheira, R., Elorza, Á. A., & Castro, A. F. (2020). Cytosolic NUAK1 enhances ATP production by maintaining proper glycolysis and mitochondrial function in cancer cells. Frontiers in Oncology, 10, 1123.

Fritz, J. L., Collins, O., Saxena, P., Buensuceso, A., Ramos Valdes, Y., Francis, K. E., ... & Shepherd, T. G. (2020). A novel role for NUAK1 in promoting ovarian cancer metastasis through regulation of fibronectin production in spheroids. Cancers, 12(5), 1250.

Palma, M., Riffo, E. N., Suganuma, T., Washburn, M. P., Workman, J. L., Pincheira, R., & Castro, A. F. (2019). Identification of a nuclear localization signal and importin beta members mediating NUAK1 nuclear import inhibited by oxidative stress. Journal of cellular biochemistry, 120(9), 16088-16107.

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For research use only. Not intended for any clinical use.

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