Mouse Anti-NUDT9 Recombinant Antibody (7A12) (CBMAB-N3927-WJ)

Name: Mouse Anti-NUDT9 Recombinant Antibody (7A12)
SKU: CBMAB-N3927-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

7A12

Application

WB, FC, IHC, IF, IHC-P

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

0.88 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Nudix Hydrolase 9

Introduction

The protein encoded by this gene belongs to the Nudix hydrolase family. Nudix boxes are found in a family of diverse enzymes that catalyze the hydrolysis of nucleoside diphosphate derivatives. This enzyme is an ADP-ribose pyrophosphatase that catalyzes the hydrolysis of ADP-ribose to AMP and ribose-5-P. It requires divalent metal ions and an intact Nudix motif for enzymatic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Entrez Gene ID

53343

UniProt ID

Q9BW91

Alternative Names

Nudix Hydrolase 9; Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 9; Adenosine Diphosphoribose Pyrophosphatase; ADP-Ribose Phosphohydrolase; ADP-Ribose Diphosphatase; ADPR-PPase; NUDT10;

Function

Hydrolyzes ADP-ribose (ADPR) to AMP and ribose 5'-phosphate.

Biological Process

ADP catabolic processIEA:Ensembl
IDP catabolic processIEA:Ensembl
Nucleobase-containing small molecule metabolic processTAS:Reactome

Cellular Location

Isoform 1
Mitochondrion

Debar, L., Ishak, L., Moretton, A., Anoosheh, S., Morel, F., Jenninger, L., ... & Farge, G. (2023). NUDT6 and NUDT9, two mitochondrial members of the NUDIX family, have distinct hydrolysis activities. Mitochondrion, 71, 93-103.

Gattkowski, E., Rutherford, T. J., Möckl, F., Bauche, A., Sander, S., Fliegert, R., & Tidow, H. (2021). Analysis of ligand binding and resulting conformational changes in pyrophosphatase NUDT9. The FEBS journal, 288(23), 6769-6782.

Gattkowski, E. (2021). Biophysical characterization of the pyrophosphatase NUDT9 and the NUDT9-homologous domain of the TRPM2 channel (Doctoral dissertation, Staats-und Universitätsbibliothek Hamburg Carl von Ossietzky).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

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Mouse Anti-NUDT9 Recombinant Antibody (7F12) (CAT#: CBMAB-N3925-WJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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