Rabbit Anti-NUP153 Recombinant Antibody (
8D4) (V2LY-0725-LY1573)

Name: Rabbit Anti-NUP153 Recombinant Antibody (8D4)
SKU: V2LY-0725-LY1573
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-NUP153 Recombinant Antibody (8D4) (V2LY-0725-LY1573) in IHC

IHC image of V2LY-0725-LY1573 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-NUP153 Recombinant Antibody (8D4) (V2LY-0725-LY1573) in IF

Immunofluorescence staining of Hela Cells with V2LY-0725-LY1573 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

8D4

Application

ELISA, IHC, IF

Immunogen

A synthesized peptide derived from human Nup153.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200
IF	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

nucleoporin 153

Entrez Gene ID

9972

UniProt ID

P49790

Function

Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with TPR, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Mediates TPR anchoring to the nuclear membrane at NPC. The repeat-containing domain may be involved in anchoring other components of the NPC to the pore membrane. Possible DNA-binding subunit of the nuclear pore complex (NPC).
(Microbial infection) Interacts with HIV-1 caspid protein P24 and thereby promotes the integration of the virus in the nucleus of non-dividing cells (in vitro).
(Microbial infection) Binds HIV-2 protein vpx and thereby promotes the nuclear translocation of the lentiviral genome (in vitro).

Biological Process

mRNA transportIEA:UniProtKB-KW
Negative regulation of RNA export from nucleusManual Assertion Based On ExperimentIDA:UniProtKB
Nuclear pore complex assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Nucleocytoplasmic transport1 PublicationIC:ComplexPortal
Protein import into nucleusManual Assertion Based On ExperimentIBA:GO_Central
RNA export from nucleusManual Assertion Based On ExperimentIBA:GO_Central
Viral entry into host cellIEA:UniProtKB-KW
Viral penetration into host nucleusIEA:UniProtKB-KW

Cellular Location

Nucleus
Nucleus membrane
Nucleus, nuclear pore complex
Tightly associated with the nuclear membrane and lamina (By similarity).
Localized to the nucleoplasmic side of the nuclear pore complex (NPC) core structure, forming a fibrous structure called the nuclear basket. Dissociates from the NPC structure early during prophase of mitosis. Integrated in the newly assembled nuclear envelope of postmitotic cells early in G1. Colocalized with NUP98 and TPR to the nuclear basket at the nucleoplasmic side of the NPC. Detected in diffuse and discrete intranuclear foci. Remained localized to the nuclear membrane after poliovirus (PV) infection.

PTM

Phosphorylated in interphase, hyperphosphorylated during mitosis. May play a role in the reversible disassembly of the nuclear pore complex during mitosis (By similarity).
Proteolytically degraded after poliovirus (PV) infection; degradation is partial and NCP- and TPR-binding domains withstand degradation.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.

Schertzer, M., Jullien, L., Pinto, A. L., Calado, R. T., Revy, P., & Londoño-Vallejo, A. (2023). Human RTEL1 Interacts with KPNB1 (Importin β) and NUP153 and Connects Nuclear Import to Nuclear Envelope Stability in S-Phase. Cells, 12(24), 2798.

Shen, Q., Kumari, S., Xu, C., Jang, S., Shi, J., Burdick, R. C., ... & Xiong, Y. (2023). The capsid lattice engages a bipartite NUP153 motif to mediate nuclear entry of HIV-1 cores. Proceedings of the National Academy of Sciences, 120(13), e2202815120.

Wang, X., Gao, H. B., Gao, Y. M., Du, S. J., & Wu, C. S. (2023). The expression and clinical significance of miR-133b and NUP153 in colorectalcancer.

Demeneva, V. V., Tolmacheva, E. N., Nikitina, T. V., Sazhenova, E. A., Yuriev, S. Y., Makhmutkhodzhaev, A. S., ... & Vasilyev, S. A. (2023). Expression of the NUP153 and YWHAB genes from their canonical promoters and alternative promoters of the LINE-1 retrotransposon in the placenta of the first trimester of pregnancy. Vavilov Journal of Genetics and Breeding, 27(1), 63.

LaJoie, D., Turkmen, A. M., Mackay, D. R., Jensen, C. C., Aksenova, V., Niwa, M., ... & Ullman, K. S. (2022). A role for Nup153 in nuclear assembly reveals differential requirements for targeting of nuclear envelope constituents. Molecular Biology of the Cell, 33(13), ar117.

Singh, S. P., Raja, S., & Mahalingam, S. (2020). Viral protein X unlocks the nuclear pore complex through a human Nup153-dependent pathway to promote nuclear translocation of the lentiviral genome. Molecular Biology of the Cell, 31(4), 304-317.

Leone, L., Colussi, C., Gironi, K., Longo, V., Fusco, S., Li Puma, D. D., ... & Grassi, C. (2019). Altered Nup153 expression impairs the function of cultured hippocampal neural stem cells isolated from a mouse model of Alzheimer’s disease. Molecular Neurobiology, 56, 5934-5949.

Wu, Y., Fang, G., Wang, X., Wang, H., Chen, W., Li, L., ... & Cai, Y. (2019). NUP153 overexpression suppresses the proliferation of colorectal cancer by negatively regulating Wnt/β-catenin signaling pathway and predicts good prognosis. Cancer Biomarkers, 24(1), 61-70.

Bilir, Ş., Kojidani, T., Mori, C., Osakada, H., Kobayashi, S., Koujin, T., ... & Haraguchi, T. (2019). Roles of Nup133, Nup153 and membrane fenestrations in assembly of the nuclear pore complex at the end of mitosis. Genes to cells, 24(5), 338-353.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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