Mouse Anti-ORC1 Recombinant Antibody (7F6/1) (CBMAB-O0017-CQ)

Name: Mouse Anti-ORC1 Recombinant Antibody (7F6/1)
SKU: CBMAB-O0017-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

7F6/1

Application

ELISA, WB, IP, ChIP

Immunogen

Bacterially expressed human ORC1 protein

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.09% Sodium Azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ORC1

Introduction

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

4998

UniProt ID

Q13415

Alternative Names

Origin Recognition Complex Subunit 1; Replication Control Protein 1; ORC1L; PARC1; Origin Recognition Complex, Subunit 1, S. Cerevisiae, Homolog-Like; Origin Recognition Complex, Subunit 1 Homolog (S. Cerevisiae); Origin Recognition Complex, Subunit 1 (Yeast Homolog)-Like

Function

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.

Biological Process

DNA replication initiationManual Assertion Based On ExperimentIBA:GO_Central
Mitotic DNA replication checkpoint signalingManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus

Involvement in disease

Meier-Gorlin syndrome 1 (MGORS1):
A syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal.

PTM

A syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal.

Wu, L., Chen, H., & Yang, C. (2023). Origin recognition complex subunit 1 (ORC1) is a potential biomarker and therapeutic target in cancer. BMC Medical Genomics, 16(1), 243.

Han, L., & Li, F. (2022). Origin recognition complex subunit 1 (ORC1) augments malignant behaviors of lung adenocarcinoma cells via targeting Wnt signaling. Bioengineered, 13(5), 13520-13533.

Ocaña-Pallarès, E., Vergara, Z., Desvoyes, B., Tejada-Jimenez, M., Romero-Jurado, A., Galván, A., ... & Gutierrez, C. (2020). Origin recognition complex (ORC) evolution is influenced by global gene duplication/loss patterns in eukaryotic genomes. Genome Biology and Evolution, 12(2), 3878-3889.

Xiong, W., Xie, C., Qiu, Y., Tu, Z., & Gong, Q. (2020). Origin recognition complex subunit 1 regulates cell growth and metastasis in glioma by altering activation of ERK and JNK signaling pathway. Molecular and cellular probes, 49, 101496.

Wang, X. K., Wang, Q. Q., Huang, J. L., Zhang, L. B., Zhou, X., Liu, J. Q., ... & Peng, T. (2020). Novel candidate biomarkers of origin recognition complex 1, 5 and 6 for survival surveillance in patients with hepatocellular carcinoma. Journal of Cancer, 11(7), 1869.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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