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Mouse Anti-OSBPL2 (AA 258-480) Recombinant Antibody (2E6) (CBMAB-O0591-CQ)

This product is a mouse antibody that recognizes OSBPL2 (AA 258-480). The antibody 2E6 can be used for immunoassay techniques such as: WB.
See all OSBPL2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2E6
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Human Recombinant protein fragment corresponding to amino acids 258-480 of human OSBPL2 (NP_653081) produced in E.coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 258-480

Target

Full Name
Oxysterol Binding Protein Like 2
Introduction
This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although the encoded protein contains only the sterol-binding domain. In vitro studies have shown that the encoded protein can bind strongly to phosphatic acid and weakly to phosphatidylinositol 3-phosphate, but cannot bind to 25-hydroxycholesterol. The protein associates with the Golgi apparatus. Transcript variants encoding different isoforms have been described.
Entrez Gene ID
9885
UniProt ID
Q9H1P3
Alternative Names
Oxysterol Binding Protein Like 2; OSBP-Related Protein 2; ORP-2; ORP2; Oxysterol-Binding Protein-Related Protein 2; Oxysterol-Binding Protein-Like 2
Function
Intracellular transport protein that binds sterols and phospholipids and mediates lipid transport between intracellular compartments. Increases plasma membrane cholesterol levels and decreases phosphatidylinositol-4,5-bisphosphate levels in the cell membrane (PubMed:30581148).
Binds phosphoinositides, such as phosphatidylinositol-4,5-bisphosphate (PubMed:30581148).
Exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate (PubMed:11279184).
Binds cholesterol, dehydroergosterol, 22(R)-hydroxycholesterol and 25-hydroxycholesterol (in vitro) (PubMed:17428193, PubMed:19224871, PubMed:30581148).
Biological Process
Bile acid biosynthetic processTAS:Reactome
Cholesterol transportManual Assertion Based On ExperimentIMP:ARUK-UCL
Intracellular cholesterol transportManual Assertion Based On ExperimentIMP:UniProtKB
Phospholipid transportManual Assertion Based On ExperimentIMP:ARUK-UCL
Plasma membrane organizationManual Assertion Based On ExperimentIMP:ARUK-UCL
Protein homotetramerizationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm, cytosol
Lipid droplet
Cell membrane
Detected on the surface of cytosolic lipid droplets (PubMed:19224871).
Recruited to the cell membrane by phosphatidylinositol-phosphate binding (PubMed:30581148).
Involvement in disease
Deafness, autosomal dominant, 67 (DFNA67):
A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Lin, K., Zhao, Y., Tang, Y., Chen, Y., Lin, M., & He, L. (2024). Collagen I-induced VCAN/ERK signaling and PARP1/ZEB1-mediated metastasis facilitate OSBPL2 defect to promote colorectal cancer progression. Cell Death & Disease, 15(1), 85.

Koh, Y. I., Oh, K. S., Kim, J. A., Noh, B., Choi, H. J., Joo, S. Y., ... & Gee, H. Y. (2022). OSBPL2 mutations impair autophagy and lead to hearing loss, potentially remedied by rapamycin. Autophagy, 18(11), 2593-2614.

Shi, H., Wang, H., Zhang, C., Lu, Y., Yao, J., Chen, Z., ... & Cao, X. (2022). Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via sonic hedgehog signaling. JCI insight, 7(4).

Wang, T., Wei, Q., Liang, L., Tang, X., Yao, J., Lu, Y., ... & Cao, X. (2020). OSBPL2 Is Required for the Binding of COPB1 to ATGL and the Regulation of Lipid Droplet Lipolysis. IScience, 23(7).

Shi, H., Wang, H., Yao, J., Lin, C., Wei, Q., Lu, Y., & Cao, X. (2020). Comparative transcriptome analysis of auditory OC-1 cells and zebrafish inner ear tissues in the absence of human OSBPL2 orthologues. Biochemical and Biophysical Research Communications, 521(1), 42-49.

Zhang, C., Zhang, H., Zhang, M., Lin, C., Wang, H., Yao, J., ... & Cao, X. (2019). OSBPL2 deficiency upregulate SQLE expression increasing intracellular cholesterol and cholesteryl ester by AMPK/SP1 and SREBF2 signalling pathway. Experimental cell research, 383(2), 111512.

Yao, J., Zeng, H., Zhang, M., Wei, Q., Wang, Y., Yang, H., ... & Dai, Y. (2019). OSBPL2-disrupted pigs recapitulate dual features of human hearing loss and hypercholesterolaemia. Journal of genetics and genomics, 46(8), 379-387.

Wu, N., Husile, H., Yang, L., Cao, Y., Li, X., Huo, W., ... & Wu, Q. (2019). A novel pathogenic variant in OSBPL2 linked to hereditary late-onset deafness in a Mongolian family. BMC Medical Genetics, 20, 1-7.

Wang, H., Lin, C., Yao, J., Shi, H., Zhang, C., Wei, Q., ... & Cao, X. (2019). Deletion of OSBPL2 in auditory cells increases cholesterol biosynthesis and drives reactive oxygen species production by inhibiting AMPK activity. Cell Death & Disease, 10(9), 627.

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For research use only. Not intended for any clinical use.

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