Mouse Anti-OTUD4 Recombinant Antibody (CBT2768) (V2LY-0625-LY438)

Name: Mouse Anti-OTUD4 Recombinant Antibody (CBT2768)
SKU: V2LY-0625-LY438
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-OTUD4 Recombinant Antibody (CBT2768) (V2LY-0625-LY438) in ELISA

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT2768

Application

WB, FC

Immunogen

Purified recombinant fragment of human OTUD4 (AA: 815-1049) expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

OTU domain containing 4

Entrez Gene ID

54726

UniProt ID

Q01804

Function

Deubiquitinase which hydrolyzes the isopeptide bond between the ubiquitin C-terminus and the lysine epsilon-amino group of the target protein (PubMed:23827681, PubMed:25944111, PubMed:29395066).
May negatively regulate inflammatory and pathogen recognition signaling in innate immune response. Upon phosphorylation at Ser-202 and Ser-204 residues, via IL-1 receptor and Toll-like receptor signaling pathway, specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators (PubMed:29395066).
Independently of the catalytic activity, acts as a scaffold for alternative deubiquitinases to assemble specific deubiquitinase-substrate complexes. Associates with USP7 and USP9X deubiquitinases to stabilize alkylation repair enzyme ALKBH3, thereby promoting the repair of alkylated DNA lesions (PubMed:25944111).

Biological Process

Innate immune responseIEA:UniProtKB-KW
Negative regulation of interleukin-1-mediated signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of toll-like receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of DNA demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein deubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Protein K48-linked deubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Protein K63-linked deubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of protein K48-linked deubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm
Nucleus
Primarily cytoplasmic.

PTM

Phosphorylated on Ser-202 and Ser-204 likely by CSNK2A1-CSNK2A2 serine/threonine-protein kinase complex. Activates 'Lys-63'-specific deubiquitinase activity.

Hu, S., Yuan, Y., Yan, R., Xie, H., Cai, R., & Xu, J. (2024). NRF2 regulates EGF stability through OTUD4 in lung adenocarcinoma. Biochemical and Biophysical Research Communications, 149798.

Ma, X., Wan, R., Wen, Y., Liu, T., Song, Y., & Zhu, Y. (2024). Deubiquitinating enzyme OTUD4 regulates metastasis in triple-negative breast cancer by stabilizing Snail1. Experimental Cell Research, 434(1), 113864.

Gao, Y., Tang, J., Ma, X., Zhang, C., Huang, L., Che, J., ... & Zhang, H. (2023). OTUD4 regulates metastasis and chemoresistance in melanoma by stabilizing Snail1. Journal of Cellular Physiology, 238(11), 2546-2555.

Yu, K., Guo, Y. Y., Liuyu, T., Wang, P., Zhang, Z. D., Lin, D., & Zhong, B. (2023). The deubiquitinase OTUD4 inhibits the expression of antimicrobial peptides in Paneth cells to support intestinal inflammation and bacterial infection. Cell Insight, 2(3), 100100.

Luo, L., Li, T., Zeng, Z., Li, H., He, X., & Chen, Y. (2023). CSE reduces OTUD4 triggering lung epithelial cell apoptosis via PAI-1 degradation. Cell Death & Disease, 14(9), 614.

Jaynes, P. W., Iyengar, P. V., Lui, S. K. L., Tan, T. Z., Vasilevski, N., Wright, S. C. E., ... & Eichhorn, P. J. A. (2020). OTUD4 enhances TGFβ signalling through regulation of the TGFβ receptor complex. Scientific Reports, 10(1), 15725.

Zhao, X., Su, X., Cao, L., Xie, T., Chen, Q., Li, J., ... & Jiang, C. (2020). OTUD4: A potential prognosis biomarker for multiple human cancers. Cancer Management and Research, 1503-1512.

Liu, H., Fan, J., Zhang, W., Chen, Q., Zhang, Y., & Wu, Z. (2020). OTUD4 alleviates hepatic ischemia-reperfusion injury by suppressing the K63-linked ubiquitination of TRAF6. Biochemical and Biophysical Research Communications, 523(4), 924-930.

Liuyu, T., Yu, K., Ye, L., Zhang, Z., Zhang, M., Ren, Y., ... & Zhong, B. (2019). Induction of OTUD4 by viral infection promotes antiviral responses through deubiquitinating and stabilizing MAVS. Cell Research, 29(1), 67-79.

Das, R., Schwintzer, L., Vinopal, S., Aguado Roca, E., Sylvester, M., Oprisoreanu, A. M., ... & Broemer, M. (2019). New roles for the de-ubiquitylating enzyme OTUD4 in an RNA–protein network and RNA granules. Journal of cell science, 132(12), jcs229252.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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