Rabbit Anti-OTUD5 Recombinant Antibody (D8Y2U) (CBMAB-CP1813-LY)

Name: Rabbit Anti-OTUD5 Recombinant Antibody (D8Y2U)
SKU: CBMAB-CP1813-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D8Y2U

Application

WB, IP

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human OTUD5 protein.

Specificity

Human, Rat, Monkey, Cattle, Dog, Pig

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

OTU Deubiquitinase 5

Introduction

This gene encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Oct 2008]

Entrez Gene ID

Human	55593
Rat	363452
Monkey	711927
Cattle	539145
Dog	480911
Pig	100624053

UniProt ID

Human	Q96G74
Rat	Q2YDU3
Monkey	A0A1D5QBJ6
Cattle	E1BFW9
Dog	F1P8I6
Pig	I3L773

Alternative Names

OTU Deubiquitinase 5; Deubiquitinating Enzyme A; OTU Domain Containing 5; DUBA; OTU Domain-Containing Protein 5; Deubiquinating Enzyme A; EC 3.4.19.12;

Function

Deubiquitinating enzyme that functions as negative regulator of the innate immune system. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro). Controls neuroectodermal differentiation through cleaving 'Lys-48'-linked ubiquitin chains to counteract degradation of select chromatin regulators such as ARID1A, HDAC2 and HCF1 (PubMed:33523931).

Biological Process

Negative regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIMP:FlyBase
Negative regulation of type I interferon productionTAS:Reactome
Neural crest cell differentiationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of TORC1 signalingManual Assertion Based On ExperimentIMP:FlyBase
Positive regulation of TORC2 signalingManual Assertion Based On ExperimentIMP:FlyBase
Protein deubiquitinationManual Assertion Based On ExperimentIDA:MGI
Protein K48-linked deubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Protein K63-linked deubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Response to lipopolysaccharideManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Nucleus

Involvement in disease

Multiple congenital anomalies-neurodevelopmental syndrome, X-linked (MCAND):
An X-linked recessive, congenital disorder characterized by central nervous system, craniofacial, cardiac, skeletal, and genitourinary anomalies. Clinical features include poor growth, short stature, global developmental delay, impaired intellectual development, microcephaly, hydrocephalus, hypotonia, congenital heart defects, hypospadias, and other variable abnormalities. Brain imaging typically shows ventriculomegaly and thin corpus callosum. The severity of the disorder is highly variable, ranging from death in early infancy to survival into the second or third decade.

PTM

Phosphorylation at Ser-177 is required for deubiquitinating activity.

Fu, L., Lu, K., Jiao, Q., Chen, X., & Jia, F. (2023). The Regulation and Double-Edged Roles of the Deubiquitinase OTUD5. Cells, 12(8), 1161.

Hou, T., Dan, W., Liu, T., Liu, B., Wei, Y., Yue, C., ... & Li, L. (2022). Deubiquitinase OTUD5 modulates mTORC1 signaling to promote bladder cancer progression. Cell Death & Disease, 13(9), 778.

Dinallo, V., Di Fusco, D., Di Grazia, A., Laudisi, F., Troncone, E., Di Maggio, G., ... & Monteleone, G. (2022). The deubiquitinating enzyme OTUD5 sustains inflammatory cytokine response in inflammatory bowel disease. Journal of Crohn's and Colitis, 16(1), 122-132.

Cho, J. H., Kim, K., Kim, S. A., Park, S., Park, B. O., Kim, J. H., ... & Kim, S. (2021). Deubiquitinase OTUD5 is a positive regulator of mTORC1 and mTORC2 signaling pathways. Cell Death & Differentiation, 28(3), 900-914.

Guo, Y., Jiang, F., Kong, L., Wu, H., Zhang, H., Chen, X., ... & Gao, C. (2021). OTUD5 promotes innate antiviral and antitumor immunity through deubiquitinating and stabilizing STING. Cellular & molecular immunology, 18(8), 1945-1955.

Beck, D. B., Basar, M. A., Asmar, A. J., Thompson, J. J., Oda, H., Uehara, D. T., ... & Werner, A. (2021). Linkage-specific deubiquitylation by OTUD5 defines an embryonic pathway intolerant to genomic variation. Science advances, 7(4), eabe2116.

Bai, M., Che, Y., Lu, K., & Fu, L. (2020). Analysis of deubiquitinase OTUD5 as a biomarker and therapeutic target for cervical cancer by bioinformatic analysis. PeerJ, 8, e9146.

Li, F., Sun, Q., Liu, K., Zhang, L., Lin, N., You, K., ... & Zhao, W. (2020). OTUD5 cooperates with TRIM25 in transcriptional regulation and tumor progression via deubiquitination activity. Nature Communications, 11(1), 4184.

De Vivo, A., Sanchez, A., Yegres, J., Kim, J., Emly, S., & Kee, Y. (2019). The OTUD5–UBR5 complex regulates FACT-mediated transcription at damaged chromatin. Nucleic acids research, 47(2), 729-746.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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