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Mouse Anti-P4HA1 Recombinant Antibody (CF325) (CBMAB-FT187LY)

The product is antibody recognizes P4HA1 . The antibody CF325 immunoassay techniques such as: ELISA, WB, IHC, IF.
See all P4HA1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CF325
Antibody Isotype
IgG1
Application
ELISA, WB, IHC, IF

Basic Information

Immunogen
prolyl 4-hydroxylase, alpha polypeptide I
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
50% glycerol
Preservative
0.02% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Prolyl 4-Hydroxylase Subunit Alpha 1
Introduction
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human5033
Mouse18451
Rat64475
UniProt ID
HumanP13674
MouseQ60715
RatP54001
Alternative Names
4 PH alpha 1, P4HA, P4HA1
Function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Biological Process
Collagen fibril organizationIEA:Ensembl
Peptidyl-proline hydroxylation to 4-hydroxy-L-prolineManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Endoplasmic reticulum lumen

Li, Y., Ge, Y. Z., Qian, Y., Chen, K., Zhao, F., Qin, Z., ... & Jia, R. (2022). The role of P4HA1 in multiple cancer types and its potential as a target in renal cell carcinoma. Frontiers in Genetics, 13, 848456.

Zhu, X., Liu, S., Yang, X., Wang, W., Shao, W., & Ji, T. (2021). P4HA1 as an unfavorable prognostic marker promotes cell migration and invasion of glioblastoma via inducing EMT process under hypoxia microenvironment. American Journal of Cancer Research, 11(2), 590.

Robinson, A. D., Chakravarthi, B. V., Agarwal, S., Chandrashekar, D. S., Davenport, M. L., Chen, G., ... & Varambally, S. (2021). Collagen modifying enzyme P4HA1 is overexpressed and plays a role in lung adenocarcinoma. Translational Oncology, 14(8), 101128.

Ning, Y., Zheng, H., Zhan, Y., Liu, S., Yang, Y., Zang, H., ... & Fan, S. (2021). Overexpression of P4HA1 associates with poor prognosis and promotes cell proliferation and metastasis of lung adenocarcinoma. Journal of Cancer, 12(22), 6685.

Zhang, Q., Yin, Y., Zhao, H., Shi, Y., Zhang, W., Yang, Z., ... & Yu, Z. (2021). P4HA1 regulates human colorectal cancer cells through HIF1α‑mediated Wnt signaling. Oncology Letters, 21(2), 1-1.

Eriksson, J., Le Joncour, V., Jahkola, T., Juteau, S., Laakkonen, P., Saksela, O., & Hölttä, E. (2020). Prolyl 4‐hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls. Molecular oncology, 14(4), 742-762.

Agarwal, S., Behring, M., Kim, H. G., Bajpai, P., Chakravarthi, B. V., Gupta, N., ... & Manne, U. (2020). Targeting P4HA1 with a small molecule inhibitor in a colorectal cancer PDX model. Translational oncology, 13(4), 100754.

Li, M., Wu, F., Zheng, Q., Wu, Y., & Wu, Y. A. (2020). Identification of potential diagnostic and prognostic values of P4HA1 expression in lung cancer, breast cancer, and head and neck cancer. DNA and Cell Biology, 39(5), 909-917.

Xu, R. (2019). P4HA1 is a new regulator of the HIF-1 pathway in breast cancer. Cell stress, 3(1), 27.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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