Mouse Anti-P4HA3 Recombinant Antibody (7F10) (CBMAB-P0613-YC)

Provided herein is a Mouse monoclonal antibody against Human Prolyl 4-Hydroxylase Subunit Alpha 3. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all P4HA3 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

7F10

Antibody Isotype

IgG2a, κ

Application

ELISA, WB

Basic Information

Immunogen

P4HA3 (NP_878907.1, 66-165 aa) partial recombinant protein with GST tag. Immunogen sequence: TRFYDKVLSL HEDSTTPVAN PLLAFTLIKR LQSDWRNVVH SLEASENIRA LKDGYEKVEQ DLPAFEDLEG AARALMRLQD VYMLNVKGLA RGVFQRVTGS

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 66-165

Target

Full Name

prolyl 4-hydroxylase, alpha polypeptide III

Introduction

P4HA3 is a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains.

Entrez Gene ID

283208

UniProt ID

Q7Z4N8

Alternative Names

Prolyl 4-Hydroxylase Subunit Alpha 3; EC 1.14.11.2; Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Alpha Polypeptide III; Procollagen-Proline,2-Oxoglutarate-4-Dioxygenase Subunit Alpha-3; Prolyl 4-Hydroxylase, Alpha Polypeptide III; Collagen Prolyl 4-Hydroxylase Alpha(III); 4-PH Alpha-3;

Function

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

Biological Process

Peptidyl-proline hydroxylation to 4-hydroxy-L-prolineManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Endoplasmic reticulum lumen

PTM

N-glycosylation plays no role in the catalytic activity.

References

Zhou, H., Zou, J., Han, J., Zhou, A., & Huang, S. (2024). P4HA3 promotes colon cancer cell escape from macrophage phagocytosis by increasing phagocytosis immune checkpoint CD47 expression. Molecular and Cellular Biochemistry, 1-20.

Zhang, Z., Zhang, Y., & Zhang, R. (2023). P4HA3 promotes clear cell renal cell carcinoma progression via the PI3K/AKT/GSK3β pathway. Medical Oncology, 40(2), 70.

Greco, F., Panunzio, A., Tafuri, A., Bernetti, C., Pagliarulo, V., Zobel, B. B., ... & Mallio, C. A. (2023). CT-based radiogenomics of P4HA3 expression in clear cell renal cell carcinoma. Academic Radiology.

Zhuang, L., Li, C., Hu, X., Yang, Q., Pei, X., & Jin, G. (2022). High expression of P4HA3 in obesity: a potential therapeutic target for type 2 diabetes. Brazilian Journal of Medical and Biological Research, 55, e11741.

Zhou, H., Huang, S., Shao, C., Zou, J., Zhou, A., Yu, J., & Xu, C. (2022). miR-1266-3p suppresses epithelial-mesenchymal transition in colon cancer by targeting P4HA3. Analytical Cellular Pathology, 2022.

Xiao, Z., Reddy, D. P. K., Xue, C., Liu, X., Chen, X., Li, J., ... & Zheng, S. (2021). Profiling of miR-205/P4HA3 following angiotensin II-induced atrial fibrosis: implications for atrial fibrillation. Frontiers in Cardiovascular Medicine, 8, 609300.

Long, R., Liu, Z., Li, J., & Yu, H. (2019). COL6A6 interacted with P4HA3 to suppress the growth and metastasis of pituitary adenoma via blocking PI3K-Akt pathway. Aging (Albany NY), 11(20), 8845.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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