Rabbit Anti-PABPN1 Recombinant Antibody (
6C3) (V2LY-0725-LY1257)

Name: Rabbit Anti-PABPN1 Recombinant Antibody (6C3)
SKU: V2LY-0725-LY1257
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-PABPN1 Recombinant Antibody (6C3) (V2LY-0725-LY1257) in WB

Western Blot
Positive WB detected in: 293 whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate
All lanes: PABPN1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 33, 32, 38 kDa
Observed band size: 50 kDa

Rabbit Anti-PABPN1 Recombinant Antibody (6C3) (V2LY-0725-LY1257) in IHC

IHC image of V2LY-0725-LY1257 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-PABPN1 Recombinant Antibody (6C3) (V2LY-0725-LY1257) in IHC

IHC image of V2LY-0725-LY1257 diluted at 1:100 and staining in paraffin-embedded human bladder cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-PABPN1 Recombinant Antibody (6C3) (V2LY-0725-LY1257) in IF

Immunofluorescence staining of Hela Cells with V2LY-0725-LY1257 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Rabbit Anti-PABPN1 Recombinant Antibody (6C3) (V2LY-0725-LY1257) in FC

Overlay histogram showing Hela cells stained with V2LY-0725-LY1257 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

6C3

Application

ELISA, WB, IHC, IF, FC

Immunogen

A synthesized peptide derived from human PABPN1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IF	1:20-1:200
FC	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Poly(A) Binding Protein Nuclear 1

Entrez Gene ID

8106

UniProt ID

Q86U42

Function

Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product (By similarity).
Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (By similarity).
Increases the affinity of poly(A) polymerase for RNA (By similarity).
Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes (PubMed:11371506).
Binds to poly(A) and to poly(G) with high affinity (By similarity).
May protect the poly(A) tail from degradation (By similarity).
Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484).

Biological Process

Cellular response to lipopolysaccharideISS:UniProtKB
MAPK cascadeISS:UniProtKB
mRNA processingIEA:UniProtKB-KW
Muscle contractionManual Assertion Based On ExperimentTAS:ProtInc
Poly(A)+ mRNA export from nucleusManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of polynucleotide adenylyltransferase activityISS:UniProtKB
RNA processingManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Nucleus
Cytoplasm
Nucleus speckle
Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus (PubMed:10688363).
Its nuclear import may involve the nucleocytoplasmic transport receptor transportin and a RAN-GTP-sensitive import mechanism (By similarity).
Is exported to the cytoplasm by a carrier-mediated pathway that is independent of mRNA traffic. Colocalizes with SKIP and poly(A) RNA in nuclear speckles (By similarity).
Intranuclear filamentous inclusions or 'aggregates' are detected in the myocytes of patients; these inclusions contain PABPN1, ubiquitin, subunits of the proteasome and poly(A) RNA.

Involvement in disease

Oculopharyngeal muscular dystrophy (OPMD):
A form of late-onset slowly progressive myopathy characterized by eyelid ptosis, dysphagia and, sometimes by other cranial and limb-muscle involvement.

PTM

Arginine dimethylation is asymmetric and involves PRMT1 and PRMT3. It does not influence the RNA binding properties (By similarity).

Wang, Q. H., Yan, P. C., Shi, L. Z., Teng, Y. J., Gao, X. J., Yao, L. Q., ... & Li, R. (2024). PABPN1 functions as a predictive biomarker in colorectal carcinoma. Molecular Biology Reports, 51(1), 40.

Kwiatek, L., Landry-Voyer, A. M., Latour, M., Yague-Sanz, C., & Bachand, F. (2023). PABPN1 prevents the nuclear export of an unspliced RNA with a constitutive transport element and controls human gene expression via intron retention. RNA, 29(5), 644-662.

Chen, L., Dong, W., Zhou, M., Yang, C., Xiong, M., Kazobinka, G., ... & Hou, T. (2023). PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression. Cell & Bioscience, 13(1), 45.

Kases, K., Schubert, E., Hajikhezri, Z., Larsson, M., Devi, P., Darweesh, M., ... & Younis, S. (2023). The RNA-binding protein ZC3H11A interacts with the nuclear poly (A)-binding protein PABPN1 and alters polyadenylation of viral transcripts. Journal of Biological Chemistry, 299(8).

Dai, X. X., Pi, S. B., Zhao, L. W., Wu, Y. W., Shen, J. L., Zhang, S. Y., ... & Fan, H. Y. (2022). PABPN1 functions as a hub in the assembly of nuclear poly (A) domains that are essential for mouse oocyte development. Science Advances, 8(43), eabn9016.

Roth, F., Dhiab, J., Boulinguiez, A., Mouigni, H. R., Lassche, S., Negroni, E., ... & Trollet, C. (2022). Assessment of PABPN1 nuclear inclusions on a large cohort of patients and in a human xenograft model of oculopharyngeal muscular dystrophy. Acta Neuropathologica, 144(6), 1157-1170.

Zhang, L., Wang, D., Han, X., Guo, X., Cao, Y., Xia, Y., & Gao, D. (2022). Novel read‐through fusion transcript Bcl2l2‐Pabpn1 in glioblastoma cells. Journal of Cellular and Molecular Medicine, 26(17), 4686-4697.

Zhao, A., & Liu, Y. (2021). Propofol suppresses colorectal cancer development by the circ-PABPN1/miR-638/SRSF1 axis. Analytical biochemistry, 631, 114354.

Mohibi, S., Zhang, J., & Chen, X. (2020). PABPN1, a target of p63, modulates keratinocyte differentiation through regulation of p63α mRNA translation. Journal of Investigative Dermatology, 140(11), 2166-2177.

Malerba, A., Klein, P., Lu-Nguyen, N., Cappellari, O., Strings-Ufombah, V., Harbaran, S., ... & Dickson, G. (2019). Established PABPN1 intranuclear inclusions in OPMD muscle can be efficiently reversed by AAV-mediated knockdown and replacement of mutant expanded PABPN1. Human Molecular Genetics, 28(19), 3301-3308.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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