Mouse Anti-PAFAH1B2 Recombinant Antibody (2F4-1C10) (CBMAB-P0656-YC)

Name: Mouse Anti-PAFAH1B2 Recombinant Antibody (2F4-1C10)
SKU: CBMAB-P0656-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2F4-1C10

Application

ELISA

Immunogen

Full length recombinant corresponding to aa1-230 from human PAFAH1B2 (AAH00398) with GST tag

Specificity

Human

Antibody Isotype

IgM, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Platelet Activating Factor Acetylhydrolase 1b Catalytic Subunit 2

Introduction

Platelet-activating factor acetylhydrolase (PAFAH) inactivates platelet-activating factor (PAF) into acetate and LYSO-PAF. This gene encodes the beta subunit of PAFAH, the other subunits are alpha and gamma. Multiple alternatively spliced transcript variants have been described for this gene.

Entrez Gene ID

5049

UniProt ID

P68402

Alternative Names

Platelet Activating Factor Acetylhydrolase 1b Catalytic Subunit 2; Platelet-Activating Factor Acetylhydrolase 1b, Catalytic Subunit 2 (30kDa); Platelet-Activating Factor Acetylhydrolase, Isoform Ib, Subunit 2 (30kDa); PAF Acetylhydrolase 30 KDa Subunit; PAF-AH1b Alpha 2 Subunit; PAF-AH 30 KDa Subunit; PAF-AH Subunit Beta; PAFAH Subunit Beta;

Function

Alpha2 catalytic subunit of the cytosolic type I platelet-activating factor (PAF) acetylhydrolase (PAF-AH (I)) heterotetrameric enzyme that catalyzes the hydrolyze of the acetyl group at the sn-2 position of PAF and its analogs and modulates the action of PAF. The activity and substrate specificity of PAF-AH (I) are affected by its subunit composition. The alpha2/alpha2 homodimer (PAFAH1B2/PAFAH1B2 homodimer) hydrolyzes PAF and 1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylethanolamine (AAGPE) more efficiently than 1-O-alkyl-2-acetyl-sn-glycero-3-phosphoric acid (AAGPA). In contrast, the alpha1/alpha2 heterodimer(PAFAH1B3/PAFAH1B3 heterodimer) hydrolyzes AAGPA more efficiently than PAF, but has little hydrolytic activity towards AAGPE (By similarity).
May play a role in male germ cell meiosis during the late pachytenestage and meiotic divisions as well as early spermiogenesis (By similarity).

Biological Process

Brain developmentManual Assertion Based On ExperimentIBA:GO_Central
Lipid catabolic processIEA:UniProtKB-KW
Lipid metabolic processManual Assertion Based On ExperimentTAS:ProtInc
Positive regulation of macroautophagyManual Assertion Based On ExperimentIMP:BHF-UCL
SpermatogenesisISS:UniProtKB

Cellular Location

Cytoplasm

Tanigawa, K., Kiriya, M., Hayashi, Y., Shinden, Y., Kijima, Y., Natsugoe, S., ... & Karasawa, K. (2022). Cathepsin G-induced malignant progression of MCF-7 cells involves suppression of PAF signaling through induced expression of PAFAH1B2. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1867(8), 159164.

Xu, Z. Y., Peng, J., Shi, Z. Z., Chen, X. L., Cheng, H. Z., Wang, H., ... & Peng, H. (2021). Silencing linc00662 inhibits cell proliferation and colony formation of lung cancer cells via regulating the miR-145-5p–PAFAH1B2 axis. Biochemistry and Cell Biology, 99(3), 330-338.

Kim, G. R., Zhao, T., Kee, H. J., Kee, S. J., & Jeong, M. H. (2021). MicroRNA-212-5p and its target PAFAH1B2 suppress vascular proliferation and contraction via the downregulation of RhoA. Plos one, 16(3), e0249146.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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