Mouse Anti-PAFAH2 Recombinant Antibody (1A8) (CBMAB-P0666-YC)

Name: Mouse Anti-PAFAH2 Recombinant Antibody (1A8)
SKU: CBMAB-P0666-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1A8

Application

ELISA

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

platelet-activating factor acetylhydrolase 2, 40kDa

Introduction

PAFAH2 is platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme.

Entrez Gene ID

5051

UniProt ID

Q99487

Alternative Names

Platelet Activating Factor Acetylhydrolase 2; Platelet-Activating Factor Acetylhydrolase 2, 40kDa; Serine-Dependent Phospholipase A2; EC 3.1.1.47; HSD-PLA2; SD-PLA2; Platelet-Activating Factor Acetylhydrolase 2, Cytoplasmic; Platelet-Activating Factor Acetylhydrolase 2 (40kD); EC 3.1.1;

Function

Catalyzes the hydrolyze of the acetyl group at the sn-2 position of platelet-activating factor (PAF) and its analogs, leading to their inactivation (PubMed:9494101).
Hydrolyzes propionyl and butyroyl moieties approximately half as effectively as PAF (By similarity).
Also catalyzes transacetylation of the acetyl group from platelet-activating factor (PAF) to lysoplasmalogen and to sphingosine, producing plasmalogen analogs of PAF and N-acetylsphingosine (C2-ceramide) respectively. Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position (By similarity).

Biological Process

Blood coagulationTAS:Reactome
Lipid catabolic processIEA:UniProtKB-KW
Lipid metabolic processManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Cytoplasm
Membrane
Endoplasmic reticulum membrane
In resting cells, localizes to intracellular membranes and cytoplasm. Translocates from the cytoplasm to intracellular membranes upon oxidative stress.

Zhang, Q., Sun, T., Yu, F., Liu, W., Gao, J., Chen, J., ... & Chen, Q. (2024). PAFAH2 suppresses synchronized ferroptosis to ameliorate acute kidney injury. Nature chemical biology, 1-12.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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