Mouse Anti-PAGE4 Recombinant Antibody (CBYC-P137) (CBMAB-P0675-YC)

Provided herein is a Mouse monoclonal antibody against Human PAGE Family Member 4. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all PAGE4 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBYC-P137

Antibody Isotype

IgG2b, κ

Application

ELISA, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.2

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

P antigen family, member 4 (prostate associated)

Introduction

PAGE4 is a member of the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in prostate and prostate cancer. It is also expressed in other male and female reproductive tissues including testis, fallopian tube, uterus, and placenta, as well as in testicular cancer and uterine cancer. The protein encoded by this gene shares sequence similarity with other GAGE/PAGE proteins, and also belongs to a family of CT (cancer-testis) antigens. The protein may play a role in benign and malignant prostate diseases. A related pseudogene is located on chromosome 7.

Entrez Gene ID

9506

UniProt ID

O60829

Alternative Names

PAGE-4; PAGE-1; GAGEC1; GAGE-9; CT16.7; JM-27

Function

Intrinsically disordered protein that potentiates the transcriptional activator activity of JUN (PubMed:24263171, PubMed:28289210).
Protects cells from stress-induced apoptosis by inhibiting reactive oxygen species (ROS) production and via regulation of the MAPK signaling pathway (PubMed:21357425, PubMed:25374899, PubMed:30658679).

Biological Process

Negative regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of oxidative stress-induced cell deathManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of reactive oxygen species biosynthetic processManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of stress-activated MAPK cascadeManual Assertion Based On ExperimentTAS:UniProtKB
Response to oxidative stressManual Assertion Based On ExperimentIDA:UniProtKB
Response to starvationManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm
Nucleus
Mitochondrion
Translocates to mitochondria in response to stress.

PTM

HIPK1-mediated phosphorylation at Thr-51 leads to the compaction of its intrinsically disordered conformation and is critical for its ability to potentiate the transcriptional activator activity of JUN inspite of a reduced interaction with JUN (PubMed:24559171, PubMed:26242913).
CLK2-mediated phosphorylation at multiple Ser and Thr residues attenuates its ability to potentiate JUN transcriptional activator activity (PubMed:28289210).

References

Li, Y., Liu, J., Liu, D., Wang, Z., Zhou, Y., Yang, S., ... & Zhang, X. (2022). The Prostate-Associated Gene 4 (PAGE4) Could Play a Role in the Development of Benign Prostatic Hyperplasia under Oxidative Stress. Oxidative Medicine and Cellular Longevity, 2022.

Singh, D., Bocci, F., Kulkarni, P., & Jolly, M. K. (2021). Coupled feedback loops involving PAGE4, EMT and notch signaling can give rise to non-genetic heterogeneity in prostate cancer cells. Entropy, 23(3), 288.

Fu, S., Liu, T., Lv, C., Fu, C., Zeng, R., Kakehi, Y., ... & Zeng, Y. (2020). Stromal‐epithelial interactions in prostate cancer: Overexpression of PAGE4 in stromal cells inhibits the invasive ability of epithelial cells. Journal of Cellular Biochemistry, 121(11), 4406-4418.

Koirala, S., Klein, J., Zheng, Y., Glenn, N. O., Eisemann, T., Tacer, K. F., ... & Potts, P. R. (2020). Tissue-specific regulation of the Wnt/β-catenin pathway by PAGE4 inhibition of tankyrase. Cell reports, 32(3).

Lin, X., Kulkarni, P., Bocci, F., Schafer, N. P., Roy, S., Tsai, M. Y., ... & Jolly, M. K. (2019). Structural and dynamical order of a disordered protein: molecular insights into conformational switching of PAGE4 at the systems level. Biomolecules, 9(2), 77.

Lv, C., Fu, S., Dong, Q., Yu, Z., Zhang, G., Kong, C., ... & Zeng, Y. (2019). PAGE4 promotes prostate cancer cells survive under oxidative stress through modulating MAPK/JNK/ERK pathway. Journal of Experimental & Clinical Cancer Research, 38, 1-15.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-PAGE4 Recombinant Antibody (7C3)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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