Sign in or Register   Sign in or Register
  |  

Mouse Anti-PAK4 Recombinant Antibody (3F10) (CBMAB-P0706-YC)

Provided herein is a Mouse monoclonal antibody against Human P21 (RAC1) Activated Kinase 4. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all PAK4 antibodies
Published Data
Fig.1 Oleate induces PAK4 phosphorylation in human islets.
...View More
Fig.2 PAK4 is recruited by Cdc42Hs to the BFA‐sensitive compartment of the Golgi.
...View More
Fig.3 Western blot analysis of the PAK4 protein level in a panel of OSCC cell lines.
...View More
Fig.4 Knock-down of PAK4 by siRNAs suppresses cell proliferation, migration, and invasion of MDA-MB-231 cells.
...View More

Summary

Host Animal
Mouse
Specificity
Human
Clone
3F10
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Immunogen
PAK4 (AAH02921, 68-157 aa) partial recombinant protein with GST tag. Immunogen sequence: KTIVRGSKGA KDGALTLLLD EFENMSVTRS NSLRRDSPPP PARARQENGM PEKPPGPRSP QREPQRVSHE QFRAALQLVV DPGDPRSYLD
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 68-157

Target

Full Name
p21 protein (Cdc42/Rac)-activated kinase 4
Introduction
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton.
Entrez Gene ID
10298
UniProt ID
O96013
Alternative Names
P21 (RAC1) Activated Kinase 4; EC 2.7.11.1; P21 Protein (Cdc42/Rac)-Activated Kinase 4; P21(CDKN1A)-Activated Kinase 4; P21-Activated Kinase 4; EC 2.7.11; KIAA1142; PAK-4;
Function
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.
Biological Process
Apoptotic processManual Assertion Based On ExperimentTAS:UniProtKB
Cell cycleIEA:UniProtKB-KW
Cell migrationManual Assertion Based On ExperimentTAS:UniProtKB
Cellular response to organic cyclic compoundIEA:Ensembl
Cytoskeleton organizationManual Assertion Based On ExperimentTAS:UniProtKB
Dendritic spine developmentIEA:Ensembl
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of endothelial cell apoptotic processManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of angiogenesisManual Assertion Based On ExperimentIMP:BHF-UCL
Protein phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of cell growthManual Assertion Based On ExperimentTAS:UniProtKB
Regulation of MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Signal transductionManual Assertion Based On ExperimentTAS:UniProtKB
Cellular Location
Cytoplasm
Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways.
PTM
Autophosphorylated on serine residues when activated by CDC42/p21 (Ref.32). Phosphorylated on tyrosine residues upon stimulation of FGFR2 (By similarity).
Methylated by SETD6.
Polyubiquitinated, leading to its proteasomal degradation.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-PAK4 Recombinant Antibody (3F10)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare