Mouse Anti-PAN2 Recombinant Antibody (6A7) (CBMAB-U0103-CQ)

Name: Mouse Anti-PAN2 Recombinant Antibody (6A7)
SKU: CBMAB-U0103-CQ
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

6A7

Antibody Isotype

IgG

Application

WB, IP, IF, ELISA

Basic Information

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

0.1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

PAN2 Poly(A) Specific Ribonuclease Subunit

Introduction

This gene encodes a deadenylase that functions as the catalytic subunit of the polyadenylate binding protein dependent poly(A) nuclease complex. The encoded protein is a magnesium dependent 3' to 5' exoribonuclease that is involved in the degradation of cytoplasmic mRNAs. Among its related pathways are Ectoderm Differentiation and Gene Expression.

Entrez Gene ID

9924

UniProt ID

Q504Q3

Alternative Names

USP52

Function

Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation.

Biological Process

mRNA processingIEA:UniProtKB-KW
Nuclear-transcribed mRNA poly(A) tail shorteningManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of cytoplasmic mRNA processing body assemblyIEA:UniProtKB-UniRule

Cellular Location

Cytoplasm
Cytoplasm, P-body 1 Automatic Annotation
Nucleus
Shuttles between nucleus and cytoplasm.

More Infomation

References

Reuter, M. S., Zech, M., Hempel, M., Altmüller, J., Heung, T., Pölsler, L., ... & Lessel, D. (2022). Biallelic PAN2 variants in individuals with a syndromic neurodevelopmental disorder and multiple congenital anomalies. European Journal of Human Genetics, 30(5), 611-618.

Tang, T. L. T. (2020). Structural and biochemical investigation of the Pan2-Pan3 deadenylase complex (Doctoral dissertation).

Tang, T. T., Stowell, J. A., Hill, C. H., & Passmore, L. A. (2019). The intrinsic structure of poly (A) RNA determines the specificity of Pan2 and Caf1 deadenylases. Nature structural & molecular biology, 26(6), 433-442.

Schäfer, I. B., Yamashita, M., Schuller, J. M., Schüssler, S., Reichelt, P., Strauss, M., & Conti, E. (2019). Molecular basis for poly (A) RNP architecture and recognition by the Pan2-Pan3 deadenylase. Cell, 177(6), 1619-1631.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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