Mouse Anti-PATJ Recombinant Antibody (15D1) (CBMAB-0641-CN)

Name: Mouse Anti-PATJ Recombinant Antibody (15D1)
SKU: CBMAB-0641-CN
Rating: 5 (1 reviews)

See all PATJ antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

15D1

Application

ELISA, MA

Immunogen

Recombinant protein fragment of INADL

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

>95%, as determined by SDS-PAGE analysis

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

PATJ, Crumbs Cell Polarity Complex Component

Introduction

This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. The protein may regulate protein targeting, cell polarity and integrity of tight junctions. It may also regulate the surface expression and/or function of ASIC3 in sensory neurons.

Entrez Gene ID

10207

UniProt ID

Q8NI35

Alternative Names

Cipp; INADL; hINADL; InaD-like

Function

Scaffolding protein that facilitates the localization of proteins to the cell membrane (PubMed:11927608, PubMed:16678097, PubMed:22006950).
Required for the correct formation of tight junctions and epithelial apico-basal polarity (PubMed:11927608, PubMed:16678097).
Positively regulates epithelial cell microtubule elongation and cell migration, possibly via facilitating localization of PRKCI/aPKC and PAR3D/PAR3 at the leading edge of migrating cells (By similarity).
Plays a role in the correct reorientation of the microtubule-organizing center during epithelial migration (By similarity).
May regulate the surface expression and/or function of ASIC3 in sensory neurons (By similarity).
May recruit ARHGEF18 to apical cell-cell boundaries (PubMed:22006950).

Biological Process

Establishment of apical/basal cell polarityISS:UniProtKB
Establishment or maintenance of epithelial cell apical/basal polarity3 PublicationsIC:ComplexPortal
Intracellular signal transduction1 PublicationNAS:UniProtKB
Microtubule organizing center organizationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of microtubule cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Tight junction assemblyISS:UniProtKB

Fiedler, J., Moennig, T., Hinrichs, J. H., Weber, A., Wagner, T., Hemmer, T., ... & Krahn, M. P. (2023). PATJ inhibits histone deacetylase 7 to control tight junction formation and cell polarity. Cellular and Molecular Life Sciences, 80(11), 333.

Owen, N., Toms, M., Tian, Y., Toualbi, L., Richardson, R., Young, R., ... & Moosajee, M. (2023). Loss of the crumbs cell polarity complex disrupts epigenetic transcriptional control and cell cycle progression in the developing retina. The Journal of Pathology, 259(4), 441-454.

Castiglioni, V. G., Ramalho, J. J., Kroll, J. R., Stucchi, R., van Beuzekom, H., Schmidt, R., ... & Boxem, M. (2022). Identification and characterization of Crumbs polarity complex proteins in Caenorhabditis elegans. Journal of Biological Chemistry, 298(4).

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-PATJ Recombinant Antibody (15D1)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry