Rabbit Anti-PCBP2 Recombinant Antibody (CBFYH-2705) (CBMAB-H3763-FY)

Name: Rabbit Anti-PCBP2 Recombinant Antibody (CBFYH-2705)
SKU: CBMAB-H3763-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBFYH-2705

Application

Specificity

Human, Mouse, Rat

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Poly(RC) Binding Protein 2

Introduction

The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

Human	5094
Mouse	18521
Rat	363005

UniProt ID

Human	Q15366
Mouse	Q61990
Rat	Q6AYU2

Alternative Names

Poly(RC) Binding Protein 2; Heterogeneous Nuclear Ribonucleoprotein E2; Heterogenous Nuclear Ribonucleoprotein E2; Poly(RC)-Binding Protein 2; Alpha-CP2; HnRNP E2; HnRNP-E2; HNRNPE2; HNRPE2

Function

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling (PubMed:19881509).
It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitination and degradation (PubMed:19881509).
(Microbial infection) In case of infection by poliovirus, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:12414943, PubMed:24371074).
Also plays a role in initiation of viral RNA replication in concert with the viral protein 3CD (PubMed:12414943).

Biological Process

Defense response to virusIEA:UniProtKB-KW
Innate immune responseIEA:UniProtKB-KW
IRES-dependent viral translational initiationManual Assertion Based On ExperimentIDA:UniProtKB
mRNA metabolic process1 PublicationNAS:UniProtKB
Negative regulation of defense response to virusManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of transcription by RNA polymerase IIIEA:Ensembl
Proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central
Regulation of RNA metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Viral RNA genome replicationManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Nucleus
Cytoplasm
Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.

PTM

Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.
(Microbial infection) Proteolyticaly cleaved by picornavirus proteinase 3CD.

Yue, L., Luo, Y., Jiang, L., Sekido, Y., & Toyokuni, S. (2022). PCBP2 knockdown promotes ferroptosis in malignant mesothelioma. Pathology international, 72(4), 242-251.

Gu, H., Yang, J., Zhang, J., Song, Y., Zhang, Y., Xu, P., ... & Sun, Q. (2022). PCBP2 maintains antiviral signaling homeostasis by regulating cGAS enzymatic activity via antagonizing its condensation. Nature Communications, 13(1), 1564.

Wang, X., Guo, Q., Wang, H., Yuan, X., Wang, B., Lobie, P. E., ... & Wu, Z. (2021). PCBP2 posttranscriptional modifications induce breast cancer progression via upregulation of UFD1 and NT5E. Molecular Cancer Research, 19(1), 86-98.

Li, Y., Zhao, Z., Lin, C. Y., Liu, Y., Staveley-OCarroll, K. F., Li, G., & Cheng, K. (2021). Silencing PCBP2 normalizes desmoplastic stroma and improves the antitumor activity of chemotherapy in pancreatic cancer. Theranostics, 11(5), 2182.

Ishii, T., Igawa, T., Hayakawa, H., Fujita, T., Sekiguchi, M., & Nakabeppu, Y. (2020). PCBP1 and PCBP2 both bind heavily oxidized RNA but cause opposing outcomes, suppressing or increasing apoptosis under oxidative conditions. Journal of Biological Chemistry, 295(34), 12247-12261.

Mao, J., Sun, Z., Cui, Y., Du, N., Guo, H., Wei, J., ... & Zheng, L. (2020). PCBP2 promotes the development of glioma by regulating FHL3/TGF‐β/Smad signaling pathway. Journal of Cellular Physiology, 235(4), 3280-3291.

Rizzotto, D., Zaccara, S., Rossi, A., Galbraith, M. D., Andrysik, Z., Pandey, A., ... & Inga, A. (2020). Nutlin-induced apoptosis is specified by a translation program regulated by PCBP2 and DHX30. Cell reports, 30(13), 4355-4369.

Wen, D., Huang, Z., Li, Z., Tang, X., Wen, X., Liu, J., & Li, M. (2020). LINC02535 co‐functions with PCBP2 to regulate DNA damage repair in cervical cancer by stabilizing RRM1 mRNA. Journal of Cellular Physiology, 235(10), 7592-7603.

Beckham, S. A., Matak, M. Y., Belousoff, M. J., Venugopal, H., Shah, N., Vankadari, N., ... & Wilce, J. A. (2020). Structure of the PCBP2/stem–loop IV complex underlying translation initiation mediated by the poliovirus type I IRES. Nucleic acids research, 48(14), 8006-8021.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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