Sign in or Register   Sign in or Register
  |  

Mouse Anti-PCBP2 Recombinant Antibody (CBYC-P200) (CBMAB-P0953-YC)

Provided herein is a Mouse monoclonal antibody against Human Poly(RC) Binding Protein 2. The antibody can be used for immunoassay techniques, such as IF, ELISA, WB.
See all PCBP2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYC-P200
Antibody Isotype
IgG2b, κ
Application
IF, ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.2
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Poly(RC) Binding Protein 2
Introduction
PCBP2 appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2).
Entrez Gene ID
5094
UniProt ID
Q15366
Alternative Names
HNRNPE2; HNRPE2; hnRNP-E2
Function
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling (PubMed:19881509).
It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitination and degradation (PubMed:19881509).
(Microbial infection) In case of infection by poliovirus, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:12414943, PubMed:24371074).
Also plays a role in initiation of viral RNA replication in concert with the viral protein 3CD (PubMed:12414943).
Biological Process
Defense response to virusIEA:UniProtKB-KW
Innate immune responseIEA:UniProtKB-KW
IRES-dependent viral translational initiationManual Assertion Based On ExperimentIDA:UniProtKB
mRNA metabolic process1 PublicationNAS:UniProtKB
Negative regulation of defense response to virusManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of transcription by RNA polymerase IIIEA:Ensembl
Proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central
Regulation of RNA metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Viral RNA genome replicationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
Cytoplasm
Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
PTM
Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.
(Microbial infection) Proteolyticaly cleaved by picornavirus proteinase 3CD.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-PCBP2 Recombinant Antibody (CBYC-P200)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare