Mouse Anti-PCGF2 (AA 236-294) Recombinant Antibody (CBFYM-3051) (CBMAB-M3247-FY)

Name: Mouse Anti-PCGF2 (AA 236-294) Recombinant Antibody (CBFYM-3051)
SKU: CBMAB-M3247-FY
Rating: 5 (1 reviews)

See all PCGF2 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-3051

Application

ELISA, WB

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 236-294

More Infomation

Target

Full Name

polycomb group ring finger 2

Introduction

The protein encoded by this gene contains a RING finger motif and is similar to the polycomb group gene products. PcG gene products form complexes via protein-protein interaction and maintain the transcription repression of genes involved in embryogenesis, cell cycles, and tumorigenesis. This protein was shown to act as a negative regulator of transcription and has tumor suppressor activity. The expression of this gene was detected in various tumor cells, but is limited in neural organs in normal tissues. Knockout studies in mice suggested that this protein may negatively regulate the expression of different cytokines, chemokines, and chemokine receptors, and thus plays an important role in lymphocyte differentiation and migration, as well as in immune responses.

Entrez Gene ID

7703

UniProt ID

P35227

Alternative Names

Polycomb Group Ring Finger 2; DNA-Binding Protein Mel-18; Ring Finger Protein 110; Zinc Finger Protein 144; RNF110; ZNF144

Function

Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells (By similarity).
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:26151332).
Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed:26151332).

Biological Process

Anterior/posterior pattern specificationIEA:Ensembl
Cellular response to hydrogen peroxideIEA:Ensembl
Embryonic skeletal system morphogenesisIEA:Ensembl
Histone acetylationIEA:Ensembl
Histone H2A-K119 monoubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
In utero embryonic developmentIEA:Ensembl
Negative regulation of apoptotic signaling pathwayIEA:Ensembl
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Nucleus

Involvement in disease

Turnpenny-Fry syndrome (TPFS):
A syndrome characterized by facial dysmorphism, intellectual disability, feeding problems, impaired growth, and a range of brain, cardiovascular, and skeletal abnormalities. Craniofacial features include frontal bossing, sparse hair, malar hypoplasia, small palpebral fissures and oral stoma, and dysplastic ears.

PTM

Phosphorylated. Homodimer formation is regulated by phosphorylation with only unphosphorylated proteins forming homodimers.

Wang, Y., Wang, W., Cheng, K., Geng, K., Liang, J., Wang, P., ... & Zhang, Y. (2022). Polycomb subunit Pcgf2 mediates ovulation and fertility through transcriptional regulation progesterone receptor. Frontiers in Cell and Developmental Biology, 10, 1010601.

Hu, J., Zhou, Y., Feng, H., Xie, Y., Qi, K., Chen, Y., ... & Cheng, B. (2022). PCGF2 and PCGF4 Oppositely Drive Stem-like Properties in Hepatocellular Carcinoma.

Qi, K. Y., Shen, M., Yang, K., Yan, Y. S., Wu, J., Wang, Y. P., & Yin, C. H. (2022). Investigation of an inherited PCGF2: p. Pro65Leu mutation causing Turnpenny-Fry syndrome. American Journal of Translational Research, 14(8), 5591.

Wang, K., Li, M., Duan, H., Fan, M., Xu, C., & Yu, F. (2022). SLCO4A1‐AS1 triggers the malignant behaviours of melanoma cells via sponging miR‐1306‐5p to enhance PCGF2. Experimental Dermatology, 31(8), 1220-1233.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-PCGF2 (AA 236-294) Recombinant Antibody (CBFYM-3051)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-PCGF2 Recombinant Antibody (2D6) (CAT#: CBMAB-A6456-LY)

Mouse Anti-PCGF2 Recombinant Antibody (3E4) (CAT#: CBMAB-A6455-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry