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Mouse Anti-PCM1 Recombinant Antibody (CL0206) (CBMAB-P1061-YC)

Provided herein is a Mouse monoclonal antibody against Human Pericentriolar Material 1. The antibody can be used for immunoassay techniques, such as WB, IHC-P.
See all PCM1 antibodies
Published Data
Fig.1 CEP215-containing protein complexes were affinity purified from CEP215-TAP, CEP215ΔHBR-TAP and CEP215ΔCM1-TAP cells, followed by western blotting for indicated antibodies.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
CL0206
Antibody Isotype
IgG1
Application
WB, IHC-P

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Pericentriolar Material 1
Introduction
PCM1 is a component of centriolar satellites, which are electron dense granules scattered around centrosomes. Inhibition studies show that this protein is essential for the correct localization of several centrosomal proteins, and for anchoring microtubules to the centrosome. Chromosomal aberrations involving this gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma.
Entrez Gene ID
5108
UniProt ID
Q15154
Alternative Names
Pericentriolar Material 1; HPCM-1; PCM-1; Pericentriolar Material 1 Protein; Pericentriolar Material 1, PCM1; RET/PCM-1; PTC4;
Function
Required for centrosome assembly and function (PubMed:12403812, PubMed:15659651, PubMed:16943179).
Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2 (PubMed:12403812, PubMed:15659651).
Required to anchor microtubules to the centrosome (PubMed:12403812, PubMed:15659651).
Also involved in cilium biogenesis by recruiting the BBSome, a ciliary protein complex involved in cilium biogenesis, to the centriolar satellites (PubMed:20551181, PubMed:24121310, PubMed:27979967).
Recruits the tubulin polyglutamylase complex (TPGC) to centriolar satellites (PubMed:34782749).
Biological Process
Centrosome cycleManual Assertion Based On ExperimentIMP:BHF-UCL
Cilium assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Cytoplasmic microtubule organizationManual Assertion Based On ExperimentIMP:UniProtKB
Interkinetic nuclear migrationISS:BHF-UCL
Intraciliary transport involved in cilium assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Microtubule anchoringISS:BHF-UCL
Microtubule anchoring at centrosomeManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of neurogenesisISS:BHF-UCL
Neuron migrationIEA:Ensembl
Neuronal stem cell population maintenanceIEA:Ensembl
Non-motile cilium assemblyManual Assertion Based On ExperimentIMP:GO_Central
Positive regulation of intracellular protein transportManual Assertion Based On ExperimentIMP:UniProtKB
Protein localization to centrosomeManual Assertion Based On ExperimentIMP:SYSCILIA_CCNET
Social behaviorIEA:Ensembl
Cellular Location
Cytoplasm, cytoskeleton
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasmic granule
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite
Cytoplasm, cytoskeleton, cilium basal body
Recruitment to the centrosome requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase. Displaced from centriolar satellites and centrosome in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, in a process that requires p38 MAP kinase signaling.
Involvement in disease
A chromosomal aberration involving PCM1 is found in papillary thyroid carcinomas (PTCs) (PubMed:10980597). Translocation t(8;10)(p21.3;q11.2) with RET links the protein kinase domain of RET to the major portion of PCM1 (PubMed:10980597).
A chromosomal aberration involving PCM1 is found in a variety of hematological malignancies including atypical chronic myeloid leukemia (atypical CML) and T-cell lymphoma (PubMed:15805263, PubMed:16034466, PubMed:16091753, PubMed:16769584, PubMed:16424865). Translocation t(8;9)(p22;p24) with JAK2 links the protein kinase domain of JAK2 to the major portion of PCM1 (PubMed:15805263, PubMed:16034466, PubMed:16091753, PubMed:16769584, PubMed:16424865).
PTM
Ubiquitinated. Undergoes monoubiquitination catalyzed by the E3 ubiquitin-protein ligase MIB1 in proliferating cells, preventing cilia formation (PubMed:24121310).
Monoubiquitination by MIB1 is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, resulting in cilia formation initiation (PubMed:24121310).
Variant Ser-159 is phosphorylated.
Phosphorylated on multiple serine and threonine residues by DYRK3 during the G2-to-M transition, after the nuclear-envelope breakdown (PubMed:29973724).
Phosphorylation by DYRK3 promotes disassembly of pericentriolar material (PubMed:29973724).
Phosphorylation at Ser-372 mediated by PLK4 is required to maintain the integrity of centriolar satellites (PubMed:30804208).
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For research use only. Not intended for any clinical use.

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