Rabbit Anti-PCYT1A Recombinant Antibody (
4F2) (V2LY-0725-LY1047)

Name: Rabbit Anti-PCYT1A Recombinant Antibody (4F2)
SKU: V2LY-0725-LY1047
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-PCYT1A Recombinant Antibody (4F2) (V2LY-0725-LY1047) in WB

Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: PCYT1A antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa

Rabbit Anti-PCYT1A Recombinant Antibody (4F2) (V2LY-0725-LY1047) in IF

Immunofluorescence staining of Hela Cells with V2LY-0725-LY1047 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

4F2

Application

ELISA, WB, IF

Immunogen

A synthesized peptide derived from human PCYT1A.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IF	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

phosphate cytidylyltransferase 1, choline, alpha

Entrez Gene ID

5130

UniProt ID

P49585

Function

Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis.

Biological Process

CDP-choline pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Phosphatidylcholine biosynthetic processManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm, cytosol
Membrane
Endoplasmic reticulum
Nucleus
It can interconvert between an inactive cytosolic form and an active membrane-bound form.

Involvement in disease

Spondylometaphyseal dysplasia with cone-rod dystrophy (SMDCRD):
A disorder characterized by postnatal growth deficiency resulting in profound short stature, rhizomelia with bowing of the lower extremities, platyspondyly with anterior vertebral protrusions, progressive metaphyseal irregularity and cupping with shortened tubular bones, and early-onset progressive visual impairment associated with a pigmentary maculopathy and electroretinographic evidence of cone-rod dysfunction.

PTM

The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation (By similarity).
Monoubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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