Mouse Anti-PDHA1 Recombinant Antibody (2C10) (CBMAB-P1280-YC)

Name: Mouse Anti-PDHA1 Recombinant Antibody (2C10)
SKU: CBMAB-P1280-YC
Rating: 5 (1 reviews)

See all PDHA1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2C10

Application

FC, IF, WB

Immunogen

A full length recombinant protein of human PDHA1 (NP_000275) produced in HEK293T cells

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Pyruvate Dehydrogenase E1 Alpha 1 Subunit

Introduction

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome.

Entrez Gene ID

5160

UniProt ID

P08559

Alternative Names

Pyruvate Dehydrogenase E1 Alpha 1 Subunit; Pyruvate Dehydrogenase E1 Component Subunit Alpha, Somatic Form, Mitochondrial; Pyruvate Dehydrogenase (Lipoamide) Alpha 1; Pyruvate Dehydrogenase Alpha 1; PDHE1-A Type I; EC 1.2.4.1; PHE1A;

Function

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.

Biological Process

Acetyl-CoA biosynthetic process from pyruvateManual Assertion Based On ExperimentIDA:UniProtKB
Glucose metabolic processIEA:UniProtKB-KW
Mitochondrial acetyl-CoA biosynthetic process from pyruvateIEA:Ensembl
Tricarboxylic acid cycleIEA:UniProtKB-KW

Cellular Location

Mitochondrion matrix

Involvement in disease

Pyruvate dehydrogenase E1-alpha deficiency (PDHAD):
An enzymatic defect causing primary lactic acidosis in children. It is associated with a broad clinical spectrum ranging from fatal lactic acidosis in the newborn to chronic neurologic dysfunction with structural abnormalities in the central nervous system without systemic acidosis.

PTM

Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation.
Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3 (By similarity).

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-PDHA1 Recombinant Antibody (2C10)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-PDHA1 Recombinant Antibody (9H9AF5) (CAT#: CBMAB-P1282-YC)

Mouse Anti-PDHA1 Recombinant Antibody (CBT4517) (CAT#: V2LY-0625-LY1988)

Mouse Anti-PDHA1 Recombinant Antibody (8D10E6) (CAT#: CBMAB-P1281-YC)

Mouse Anti-PDHA1 Recombinant Antibody (CB05A) (CAT#: CBMAB-CA005LY)

Mouse Anti-PDHA1 Recombinant Antibody (CBT3471) (CAT#: V2LY-0625-LY1989)

Mouse Anti-PDHA1 Recombinant Antibody (2B10) (CAT#: CBMAB-P1278-YC)

Mouse Anti-PDHA1 Recombinant Antibody (CBT3345) (CAT#: V2LY-0625-LY1920)

Mouse Anti-PDHA1 Recombinant Antibody (CF333) (CAT#: CBMAB-FT168LY)

Mouse Anti-PDHA1 Recombinant Antibody (CBT1012) (CAT#: V2LY-0625-LY3110)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry