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Rabbit Anti-PELP1 Recombinant Antibody (D5Q4W) (CBMAB-P1442-YC)

Provided herein is a Rabbit monoclonal antibody against Human Proline, Glutamate And Leucine Rich Protein 1. The antibody can be used for immunoassay techniques, such as IHC, IP, WB.
See all PELP1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
D5Q4W
Antibody Isotype
IgG
Application
IHC, IP, WB

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding Gly966 of human PELP1
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid in 10 mM, pH 7.5, 100 µg/ml BSA, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Proline, Glutamate And Leucine Rich Protein 1
Introduction
PELP1 is a transcription factor which coactivates transcription of estrogen receptor responsive genes and corepresses genes activated by other hormone receptors or sequence-specific transcription factors. Expression of this gene is regulated by both members of the estrogen receptor family. This gene may be involved in the progression of several types of cancer.
Entrez Gene ID
Human27043
Mouse75273
Rat360552
UniProt ID
HumanQ8IZL8
MouseQ9DBD5
RatQ56B11
Alternative Names
Proline, Glutamate And Leucine Rich Protein 1; Modulator Of Non-Genomic Activity Of Estrogen Receptor; Transcription Factor HMX3; MNAR; Proline-, Glutamic Acid-And Leucine-Rich Protein 1; Proline, Glutamic Acid And Leucine Rich Protein 1; Proline And Glutamic Acid Rich Nuclear Protein; P160; HMX3;
Function
Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors (PubMed:14963108).
Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Functions as the key stabilizing component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. Component of the PELP1 complex involved in the nucleolar steps of 28S rRNA maturation and the subsequent nucleoplasmic transit of the pre-60S ribosomal subunit. Regulates pre-60S association of the critical remodeling factor MDN1 (PubMed:21326211).
May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K.
Biological Process
Cellular response to estrogen stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus, nucleolus
Nucleus, nucleoplasm
Nucleus
Cytoplasm
Mainly found in the nucleoplasm, with low levels detected in the cytoplasm (By similarity).
Also found associated with the plasma membrane. Mainly in cytoplasm in a subset of breast tumors. Localization is widely deregulated in endometrial cancers with predominantly cytoplasm localization in high-grade endometrial tumors (PubMed:16140940).
PTM
Transiently sumoylated, preferentially conjugated to SUMO2 or SUMO3. Sumoylation causes nucleolar exclusion of PELP1 and promotes the recruitment of MDN1 to pre-60S particles. Desumoylation by SUMO isopeptidase SENP3 is needed to release both PELP1 and MDN1 from pre-ribosomes.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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