Sign in or Register   Sign in or Register
  |  

Mouse Anti-PHF1 Recombinant Antibody (2D3) (CBMAB-P1651-YC)

Provided herein is a Mouse monoclonal antibody against Human PHD Finger Protein 1. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all PHF1 antibodies
Published Data
Fig.1 Neuropathology in lumbar spinal cord of FAD-linked PS1ΔE9 mice.
...View More

Summary

Host Animal
Mouse
Specificity
Human
Clone
2D3
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Immunogen
PHF1 (NP_077084, 2-100 aa) partial recombinant protein with GST tag. Immunogen sequence: AQPPRLSRSG ASSLWDPASP APTSGPRPRL WEGQDVLARW TDGLLYLGTI KKVDSAREVC LVQFEDDSQF LVLWKDISPA ALPGEELLCC VCRSETVVP
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 2-100

Target

Full Name
PHD finger protein 1
Introduction
PHF1 is a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination.
Entrez Gene ID
5252
UniProt ID
O43189
Alternative Names
PHD Finger Protein 1; Tudor Domain Containing 19C; Polycomb-Like Protein 1; Polycomb-Like 1; HPCl1; HPHF1; PCL1;
Function
Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (PubMed:18285464 and PubMed:23273982).
According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2 (PubMed:23142980).
Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53.
Biological Process
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Chromatin organizationManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of histone H3-K27 methylationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of histone H3-K27 methylationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Localizes specifically to the promoters of numerous target genes. Localizes to double-strand breaks (DSBs) sites following DNA damage. Co-localizes with NEK6 in the centrosome.
Involvement in disease
A chromosomal aberration involving PHF1 may be a cause of endometrial stromal tumors. Translocation t(6;7)(p21;p22) with JAZF1. Translocation t(1;6)(p34;p21) with MEAF6.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-PHF1 Recombinant Antibody (2D3)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare