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Recombinant Rabbit Anti-Phospho-AKT1-S473 Antibody (CBAb256) (CBMAB-Ab256-LY)

The product is antibody recognizes Phospho-AKT1-S473. The antibody immunoassay techniques such as: WB 1:500-1:2000; IHC 1:50-1:200; IF 1:100-1:500.
See all Phospho-AKT1-S473 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
CBAb256
Antibody Isotype
IgG
Application
WB 1:500-1:2000; IHC 1:50-1:200; IF 1:100-1:500

Basic Information

Immunogen
A phospho specific peptide corresponding to residues surrounding S473 of human Akt1
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Introduction
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
Entrez Gene ID
Human207
Mouse11651
Rat24185
UniProt ID
HumanP31749
MouseP31750
RatP47196
Alternative Names
AKT1; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT serine/threonine kinase 1
Function
207
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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