Rabbit Anti-Histone H3.3 (Phospho-Thr3) Recombinant Antibody (
28H4) (V2LY-0725-LY743)

Name: Rabbit Anti-Histone H3.3 (Phospho-Thr3) Recombinant Antibody (28H4)
SKU: V2LY-0725-LY743
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-Histone H3.3 (Phospho-Thr3) Recombinant Antibody (28H4) (V2LY-0725-LY743) in WB

Western Blot
Positive WB detected in:Hela whole cell lysate,293 whole cell lysate,NIH/3T3 whole cell lysate
All lanes:Phospho-Histone H3 (T3) antibody at 1.41μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 KDa
Observed band size: 16 KDa

Rabbit Anti-Histone H3.3 (Phospho-Thr3) Recombinant Antibody (28H4) (V2LY-0725-LY743) in ICC

Immunocytochemistry analysis of V2LY-0725-LY743 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Rabbit Anti-Histone H3.3 (Phospho-Thr3) Recombinant Antibody (28H4) (V2LY-0725-LY743) in FC

Overlay histogram showing Hela cells stained with V2LY-0725-LY743 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

28H4

Application

ELISA, WB, ICC, FC

Immunogen

A synthesized peptide.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
ICC	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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