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Rabbit Anti-Phospho-MAPKAP1 (Thr86) Recombinant Antibody (D4U9L) (CBMAB-CP3064-LY)

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Basic Information

Host Animal
Rabbit
Clone
D4U9L
Application
WB, IP
Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding phosphorylated Thr86 of human Sin1 protein.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Mitogen-Activated Protein Kinase Associated Protein 1
Introduction
This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human79109
Mouse227743
Rat296648
UniProt ID
HumanQ9BPZ7
MouseQ8BKH7
RatQ6AYF1
Alternative Names
Mitogen-Activated Protein Kinase Associated Protein 1; Mitogen-Activated Protein Kinase 2-Associated Protein 1; Stress-Activated Protein Kinase-Interacting 1; SAPK-Interacting Protein 1; TORC2 Subunit MAPKAP1; MSIN1; MIP1; SIN1; Stress-Activated Map Kinase Interacting Protein 1;
Function
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.
Biological Process
Cellular response to nutrient levels1 PublicationIC:ComplexPortal
Cytoskeleton organization1 PublicationIC:ComplexPortal
Negative regulation of apoptotic process1 PublicationIC:ComplexPortal
Negative regulation of Ras protein signal transductionManual Assertion Based On ExperimentIMP:UniProtKB
PhosphorylationManual Assertion Based On ExperimentIDA:ComplexPortal
Positive regulation of cell growth1 PublicationIC:ComplexPortal
Positive regulation of peptidyl-serine phosphorylationIEA:Ensembl
Regulation of cellular response to oxidative stressIEA:Ensembl
Substantia nigra developmentManual Assertion Based On ExperimentHEP:UniProtKB
TORC2 signalingManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cell membrane
Cytoplasmic vesicle
Nucleus
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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