Rabbit Anti-STAT3 (Phospho-Tyr705) Recombinant Antibody (
3D11) (V2LY-0725-LY888)

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Tested Data
Rabbit Anti-STAT3 (Phospho-Tyr705) Recombinant Antibody (3D11) (V2LY-0725-LY888) in WB
Rabbit Anti-STAT3 (Phospho-Tyr705) Recombinant Antibody (3D11) (V2LY-0725-LY888) in WB
Western Blot
Positive WB detected in A549 whole cell lysate,Jurkat whole cell lysate(treated with EGF or Pervanadate)
All lanes Phospho-STAT3 antibody at 0.925μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 88 KDa
Observed band size: 88 KDa
Rabbit Anti-STAT3 (Phospho-Tyr705) Recombinant Antibody (3D11) (V2LY-0725-LY888) in IHC
Rabbit Anti-STAT3 (Phospho-Tyr705) Recombinant Antibody (3D11) (V2LY-0725-LY888) in IHC
IHC image of V2LY-0725-LY888 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Datasheet
SDS
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Basic Information

Host Animal
Rabbit
Clone
3D11
Application
ELISA, WB, IHC
Immunogen
A synthesized peptide derived from Human Phospho-STAT3 (Tyr705).
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:5,000
IHC1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, glycerol
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
signal transducer and activator of transcription 3
Entrez Gene ID
6774
Alternative Names
Acute phase response factor, APRF, FLJ20882, HIES, STAT3
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-STAT3 (Phospho-Tyr705) Recombinant Antibody (EC213) (CAT#: V2LY-0624-LY213)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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