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Mouse Anti-PIAS1 Recombinant Antibody (4A4) (CBMAB-P1736-YC)

Provided herein is a Mouse monoclonal antibody against Human Protein Inhibitor Of Activated STAT 1. The antibody can be used for immunoassay techniques, such as ELISA, PLA, WB.
See all PIAS1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4A4
Antibody Isotype
IgG2b, κ
Application
ELISA, PLA, WB

Basic Information

Immunogen
PIAS1 (NP_057250, 543-651 aa) partial recombinant protein with GST tag. Immunogen sequence: LDFFPFLSGD NQHYNTSLLA AAAAAVSDDQ DLLHSSRFFP YTSSQMFLDQ LSAGGSTSLP TTNGSSSGSN SSLVSSNSLR ESHSHTVTNR SSTDTASIFG IIPDIISLD
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 543-651

Target

Full Name
Protein Inhibitor Of Activated STAT 1
Introduction
PIAS1 is a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways.
Entrez Gene ID
8554
UniProt ID
O75925
Alternative Names
Protein Inhibitor Of Activated STAT 1; DEAD/H (Asp-Glu-Ala-Asp/His) Box Binding Protein 1; Protein Inhibitor Of Activated STAT Protein 1; Zinc Finger, MIZ-Type Containing 3; RNA Helicase II-Binding Protein; DEAD/H Box-Binding Protein 1; Gu-Binding Protein; DDXBP1;
Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity).
Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity).
Biological Process
Fat cell differentiationIEA:Ensembl
Negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Positive regulation of proteasomal ubiquitin-dependent protein catabolic processISS:UniProtKB
Positive regulation of protein sumoylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein sumoylationManual Assertion Based On ExperimentIDA:BHF-UCL
Receptor signaling pathway via JAK-STATManual Assertion Based On ExperimentTAS:ProtInc
Regulation of cell population proliferationISS:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus speckle
Nucleus, PML body
Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
PTM
Sumoylated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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