Mouse Anti-PIAS2 Recombinant Antibody (1F7) (CBMAB-P1740-YC)

Name: Mouse Anti-PIAS2 Recombinant Antibody (1F7)
SKU: CBMAB-P1740-YC
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

1F7

Application

ELISA, IF, PLA, RNAi, WB

Immunogen

PIAS2 (NP_004662, 385-473 aa) partial recombinant protein with GST tag. Immunogen sequence: CPVCDKKAAY ESLILDGLFM EILNDCSDVD EIKFQEDGSW CPMRPKKEAM KVSSQPCTKI ESSSVLSKPC SVTVASEASK KKVDVIDLT

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 385-473

More Infomation

Target

Full Name

Protein Inhibitor Of Activated STAT 2

Introduction

PIAS2 is a member of the protein inhibitor of activated STAT family, which function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1.

Entrez Gene ID

9063

UniProt ID

O75928

Alternative Names

Protein Inhibitor Of Activated STAT 2; Androgen Receptor-Interacting Protein 3; Protein Inhibitor Of Activated STAT X; Msx-Interacting Zinc Finger Protein; Zinc Finger, MIZ-Type Containing 4; DAB2-Interacting Protein; ARIP3; PIASX; MIZ1;

Function

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.

Biological Process

Negative regulation of androgen receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of DNA-binding transcription factor activityIDA:ParkinsonsUK-UCL
Protein sumoylationManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Transcription, DNA-templatedIEA:Ensembl

Cellular Location

Nucleus speckle
Nucleus, PML body
Nucleus
Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) (PubMed:22406621).
Colocalizes with SUMO1 in nuclear granules (By similarity).

PTM

Sumoylated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

PIAS2 Matched Antibody Pair (876) (CAT#: APMAB-876LY)

Related Products

PIAS2 Matched Antibody Pair (876) (CAT#: APMAB-876LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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