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Mouse Anti-PIAS2 Recombinant Antibody (1F7) (CBMAB-P1740-YC)

Provided herein is a Mouse monoclonal antibody against Human Protein Inhibitor Of Activated STAT 2. The antibody can be used for immunoassay techniques, such as ELISA, IF, PLA, RNAi, WB.
See all PIAS2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1F7
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, PLA, RNAi, WB

Basic Information

Immunogen
PIAS2 (NP_004662, 385-473 aa) partial recombinant protein with GST tag. Immunogen sequence: CPVCDKKAAY ESLILDGLFM EILNDCSDVD EIKFQEDGSW CPMRPKKEAM KVSSQPCTKI ESSSVLSKPC SVTVASEASK KKVDVIDLT
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 385-473

Target

Full Name
Protein Inhibitor Of Activated STAT 2
Introduction
PIAS2 is a member of the protein inhibitor of activated STAT family, which function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1.
Entrez Gene ID
9063
UniProt ID
O75928
Alternative Names
Protein Inhibitor Of Activated STAT 2; Androgen Receptor-Interacting Protein 3; Protein Inhibitor Of Activated STAT X; Msx-Interacting Zinc Finger Protein; Zinc Finger, MIZ-Type Containing 4; DAB2-Interacting Protein; ARIP3; PIASX; MIZ1;
Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.
Biological Process
Negative regulation of androgen receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of DNA-binding transcription factor activityIDA:ParkinsonsUK-UCL
Protein sumoylationManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Transcription, DNA-templatedIEA:Ensembl
Cellular Location
Nucleus speckle
Nucleus, PML body
Nucleus
Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) (PubMed:22406621).
Colocalizes with SUMO1 in nuclear granules (By similarity).
PTM
Sumoylated.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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