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Rabbit Anti-PIAS4 Recombinant Antibody (D2F12) (CBMAB-P1745-YC)

Provided herein is a Rabbit monoclonal antibody against Human Protein Inhibitor Of Activated STAT 4. The antibody can be used for immunoassay techniques, such as WB.
See all PIAS4 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Rat, Monkey, Mouse
Clone
D2F12
Antibody Isotype
IgG
Application
WB

Basic Information

Specificity
Human, Rat, Monkey, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Protein Inhibitor Of Activated STAT 4
Introduction
PIAS4 has broad expression in testis (RPKM 14.8), spleen (RPKM 3.8) and 25 other tissues.
Entrez Gene ID
Human51588
Mouse59004
Rat362827
Monkey714328
UniProt ID
HumanQ8N2W9
MouseQ9JM05
RatB5DFF9
MonkeyA0A5F7ZGF4
Alternative Names
Protein Inhibitor Of Activated STAT 4; Protein Inhibitor Of Activated STAT Protein Gamma; Protein Inhibitor Of Activated STAT Protein 4; RING-Type E3 Ubiquitin Transferase PIAS4; Zinc Finger, MIZ-Type Containing 6; PIAS-Gamma; PIASY;
Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor (PubMed:12511558, PubMed:12631292, PubMed:12727872, PubMed:15831457, PubMed:15976810, PubMed:22508508, PubMed:32832608).
Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168 (PubMed:12511558, PubMed:12631292, PubMed:12727872, PubMed:15831457, PubMed:15976810, PubMed:22508508).
Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway (PubMed:11388671).
Involved in gene silencing (PubMed:11248056).
In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations (PubMed:12727872, PubMed:15831457).
Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678).
Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs) (By similarity).
Catalyzes conjugation of SUMO2 to KAT5 in response to DNA damage, facilitating repair of DNA double-strand breaks (DSBs) via homologous recombination (HR) (PubMed:32832608).
Biological Process
Central nervous system developmentIEA:Ensembl
Double-strand break repairTAS:Reactome
Hair follicle developmentIEA:Ensembl
Limb epidermis developmentIEA:Ensembl
Negative regulation of NF-kappaB transcription factor activityIEA:Ensembl
Negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of tumor necrosis factor-mediated signaling pathwayIEA:Ensembl
Positive regulation of double-strand break repair via homologous recombinationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of intrinsic apoptotic signaling pathway in response to DNA damageIEA:Ensembl
Positive regulation of keratinocyte apoptotic processISS:BHF-UCL
Positive regulation of protein sumoylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein sumoylationManual Assertion Based On ExperimentIDA:BHF-UCL
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Vitamin D metabolic processTAS:Reactome
Wnt signaling pathwayIEA:UniProtKB-KW
Cellular Location
Nucleus, PML body
Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.
PTM
Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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