Mouse Anti-PIGT Recombinant Antibody (2A2) (CBMAB-P1771-YC)

Name: Mouse Anti-PIGT Recombinant Antibody (2A2)
SKU: CBMAB-P1771-YC
Rating: 5 (1 reviews)

See all PIGT antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2A2

Application

ELISA

Immunogen

PIGT (NP_057021.2, 24-123 aa) partial recombinant protein with GST tag. Immunogen sequence: EPPRDSLREE LVITPLPSGD VAATFQFRTR WDSELQREGV SHYRLFPKAL GQLISKYSLR ELHLSFTQGF WRTRYWGPPF LQAPSGAELW VWFQDTVTDV

Specificity

Human

Antibody Isotype

IgG2a, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 24-123

More Infomation

Target

Full Name

phosphatidylinositol glycan anchor biosynthesis, class T

Introduction

PIGT is a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.

Entrez Gene ID

51604

UniProt ID

Q969N2

Alternative Names

Phosphatidylinositol Glycan Anchor Biosynthesis Class T; Phosphatidylinositol-Glycan Biosynthesis Class T Protein; GPI Transamidase Subunit; Neurotrophin-Regulated Neuronal Development-Associated Protein; Phosphatidylinositol Glycan, Class T; GPI Transamidase Component PIG-T;

Function

Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.

Biological Process

Attachment of GPI anchor to proteinManual Assertion Based On ExperimentIBA:GO_Central
Neuron apoptotic processIEA:Ensembl
Neuron differentiationIEA:Ensembl

Cellular Location

Endoplasmic reticulum membrane

Involvement in disease

Multiple congenital anomalies-hypotonia-seizures syndrome 3 (MCAHS3):
An autosomal recessive syndrome characterized by distinct facial features, intellectual disability, hypotonia and seizures, in combination with abnormal skeletal, endocrine, and ophthalmologic findings including impaired vision, as well as abnormal motility of the eyes.
Paroxysmal nocturnal hemoglobinuria 2 (PNH2):
A disorder characterized by hemolytic anemia with hemoglobinuria, thromboses in large vessels, and a deficiency in hematopoiesis. Red blood cell breakdown with release of hemoglobin into the urine is manifested most prominently by dark-colored urine in the morning.

Topology

Lumenal: 22-527
Helical: 528-548
Cytoplasmic: 549-578

PTM

The disulfide bond between PIGK/GPI8 and PIGT is important for normal enzyme activity.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-PIGT Recombinant Antibody (2A2)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry