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Mouse Anti-PITRM1 Recombinant Antibody (1H3) (CBMAB-P1897-YC)

Provided herein is a Mouse monoclonal antibody against Human Pitrilysin Metallopeptidase 1. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all PITRM1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1H3
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
PITRM1 (AAH01150, 1-534 aa) full length recombinant protein with GST tag. Immunogen sequence: MRPDDKYHEK QAQVEATKLK QKVEALSPGD RQQIYEKGLE LRSQQSKPQD ASCLPALKVS DIEPTIPVTE LDVVLTAGDI PVQYCAQPTN GMVYFRAFSS LNTLPEELRP YVPLFCSVLT KLGCGLLDYR EQAQQIELKT GGMSASPHVL PDDSHMDTYE QGVLFSSLCL DRNLPDMMQL WSEIFNNPCF EEEEHFKVLV KMTAQELANG IPDSGHLYAS IRAGRTLTPA GDLQETFSGM DQVRLMKRIA EMTDIKPILR KLPRIKKHLL NGDNMRCSVN ATPQQMPQTE KAVEDFLRSI GRSKKERRPV RPHTVEKPVP SSSGGDAHVP HGSQVIRKLV MEPTFKPWQM KTHFLMPFPV NYVGECIRTV PYTDPDHASL KILARLMTAK FLHTEIREKG GAYGGGAKLS HNGIFTLYSY RDPNTIETLQ SFGKAVDWAK SGKFTQQDID EAKLSVFSTV DAPVAPSDKG MDHFLYGLSD EMKQAHREQL FAVSHDKLLA VSDRYLGTGK STHGLAILGP ENPKIAKDPS WIIR
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-534

Target

Full Name
pitrilysin metallopeptidase 1
Introduction
PITRM1 is an ATP-dependent metalloprotease that degrades post-cleavage mitochondrial transit peptides. The encoded protein binds zinc and can also degrade amyloid beta A4 protein, suggesting a possible role in Alzheimer's disease.
Entrez Gene ID
UniProt ID
Alternative Names
PreP; MP1
Function
Metalloendopeptidase of the mitochondrial matrix that functions in peptide cleavage and degradation rather than in protein processing (PubMed:10360838, PubMed:16849325, PubMed:19196155, PubMed:24931469).
Has an ATP-independent activity (PubMed:16849325).
Specifically cleaves peptides in the range of 5 to 65 residues (PubMed:19196155).
Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference (PubMed:10360838, PubMed:19196155, PubMed:24931469).
Degrades the transit peptides of mitochondrial proteins after their cleavage (PubMed:19196155).
Also degrades other unstructured peptides (PubMed:19196155).
It is also able to degrade amyloid-beta protein 40, one of the peptides produced by APP processing, when it accumulates in mitochondrion (PubMed:16849325, PubMed:24931469, PubMed:26697887).
It is a highly efficient protease, at least toward amyloid-beta protein 40 (PubMed:24931469, PubMed:29764912, PubMed:29383861).
Cleaves that peptide at a specific position and is probably not processive, releasing digested peptides intermediates that can be further cleaved subsequently (PubMed:24931469).
It is also able to degrade amyloid-beta protein 42 (PubMed:29764912).
Biological Process
Protein processingManual Assertion Based On ExperimentIBA:GO_Central
Protein targeting to mitochondrionTAS:Reactome
ProteolysisManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Mitochondrion
Mitochondrion matrix
Involvement in disease
Spinocerebellar ataxia, autosomal recessive, 30 (SCAR30):
A form of spinocerebellar ataxia, a clinically and genetically heterogeneous group of cerebellar disorders due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR30 is a progressive disease characterized by childhood-onset global developmental delay with variably impaired intellectual development, motor dysfunction, and cerebellar ataxia. Affected individuals may also have psychiatric abnormalities.
PTM
A disulfide bond locks the enzyme in the closed conformation preventing substrate entry into the catalytic chamber.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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