Mouse Anti-PITRM1 Recombinant Antibody (1H3) (CBMAB-P1897-YC)

Name: Mouse Anti-PITRM1 Recombinant Antibody (1H3)
SKU: CBMAB-P1897-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

1H3

Application

ELISA, WB

Immunogen

PITRM1 (AAH01150, 1-534 aa) full length recombinant protein with GST tag. Immunogen sequence: MRPDDKYHEK QAQVEATKLK QKVEALSPGD RQQIYEKGLE LRSQQSKPQD ASCLPALKVS DIEPTIPVTE LDVVLTAGDI PVQYCAQPTN GMVYFRAFSS LNTLPEELRP YVPLFCSVLT KLGCGLLDYR EQAQQIELKT GGMSASPHVL PDDSHMDTYE QGVLFSSLCL DRNLPDMMQL WSEIFNNPCF EEEEHFKVLV KMTAQELANG IPDSGHLYAS IRAGRTLTPA GDLQETFSGM DQVRLMKRIA EMTDIKPILR KLPRIKKHLL NGDNMRCSVN ATPQQMPQTE KAVEDFLRSI GRSKKERRPV RPHTVEKPVP SSSGGDAHVP HGSQVIRKLV MEPTFKPWQM KTHFLMPFPV NYVGECIRTV PYTDPDHASL KILARLMTAK FLHTEIREKG GAYGGGAKLS HNGIFTLYSY RDPNTIETLQ SFGKAVDWAK SGKFTQQDID EAKLSVFSTV DAPVAPSDKG MDHFLYGLSD EMKQAHREQL FAVSHDKLLA VSDRYLGTGK STHGLAILGP ENPKIAKDPS WIIR

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 1-534

More Infomation

Target

Full Name

pitrilysin metallopeptidase 1

Introduction

PITRM1 is an ATP-dependent metalloprotease that degrades post-cleavage mitochondrial transit peptides. The encoded protein binds zinc and can also degrade amyloid beta A4 protein, suggesting a possible role in Alzheimer's disease.

Entrez Gene ID

10531

UniProt ID

Q5JRX3

Alternative Names

PreP; MP1

Function

Metalloendopeptidase of the mitochondrial matrix that functions in peptide cleavage and degradation rather than in protein processing (PubMed:10360838, PubMed:16849325, PubMed:19196155, PubMed:24931469).
Has an ATP-independent activity (PubMed:16849325).
Specifically cleaves peptides in the range of 5 to 65 residues (PubMed:19196155).
Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference (PubMed:10360838, PubMed:19196155, PubMed:24931469).
Degrades the transit peptides of mitochondrial proteins after their cleavage (PubMed:19196155).
Also degrades other unstructured peptides (PubMed:19196155).
It is also able to degrade amyloid-beta protein 40, one of the peptides produced by APP processing, when it accumulates in mitochondrion (PubMed:16849325, PubMed:24931469, PubMed:26697887).
It is a highly efficient protease, at least toward amyloid-beta protein 40 (PubMed:24931469, PubMed:29764912, PubMed:29383861).
Cleaves that peptide at a specific position and is probably not processive, releasing digested peptides intermediates that can be further cleaved subsequently (PubMed:24931469).
It is also able to degrade amyloid-beta protein 42 (PubMed:29764912).

Biological Process

Protein processingManual Assertion Based On ExperimentIBA:GO_Central
Protein targeting to mitochondrionTAS:Reactome
ProteolysisManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Mitochondrion
Mitochondrion matrix

Involvement in disease

Spinocerebellar ataxia, autosomal recessive, 30 (SCAR30):
A form of spinocerebellar ataxia, a clinically and genetically heterogeneous group of cerebellar disorders due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR30 is a progressive disease characterized by childhood-onset global developmental delay with variably impaired intellectual development, motor dysfunction, and cerebellar ataxia. Affected individuals may also have psychiatric abnormalities.

PTM

A disulfide bond locks the enzyme in the closed conformation preventing substrate entry into the catalytic chamber.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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