Mouse Anti-PKD2L1 Recombinant Antibody (4F9) (CBMAB-P1921-YC)

Name: Mouse Anti-PKD2L1 Recombinant Antibody (4F9)
SKU: CBMAB-P1921-YC
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

4F9

Application

ELISA, WB

Immunogen

PKD2L1 (NP_057196.2, 173-272 aa) partial recombinant protein with GST tag. Immunogen sequence: KWYNNQSLGH GSHSFIYYEN MLLGVPRLRQ LKVRNDSCVV HEDFREDILS CYDVYSPDKE EQLPFGPFNG TAWTYHSQDE LGGFSHWGRL TSYSGGGYYL

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 173-272

More Infomation

Target

Full Name

polycystic kidney disease 2-like 1

Introduction

PKD2L1 is a member of the polycystin protein family. The encoded protein contains multiple transmembrane domains, and cytoplasmic N-and C-termini. The protein may be an integral membrane protein involved in cell-cell/matrix interactions. This protein functions as a calcium-regulated nonselective cation channel.

Entrez Gene ID

9033

UniProt ID

Q9P0L9

Alternative Names

TRPP3; PCL; PKD2L; PKDL

Function

Pore-forming subunit of a heterotetrameric, non-selective cation channel that is permeable to Ca2+ (PubMed:10517637, PubMed:11959145, PubMed:25820328, PubMed:27754867, PubMed:29425510, PubMed:23212381, PubMed:30004384).
Pore-forming subunit of a calcium-permeant ion channel formed by PKD1L2 and PKD1L1 in primary cilia, where it controls cilium calcium concentration, but does not affect cytoplasmic calcium concentration (PubMed:24336289).
The channel formed by PKD1L2 and PKD1L1 in primary cilia regulates sonic hedgehog/SHH signaling and GLI2 transcription (PubMed:24336289).
Pore-forming subunit of a channel formed by PKD1L2 and PKD1L3 that contributes to sour taste perception in gustatory cells (PubMed:19812697).
The heteromeric channel formed by PKD1L2 and PKD1L3 is activated by low pH, but opens only when the extracellular pH rises again (PubMed:23212381).
May play a role in the perception of carbonation taste (By similarity).
May play a role in the sensory perception of water, via a mechanism that activates the channel in response to dilution of salivary bicarbonate and changes in salivary pH (By similarity).

Biological Process

Cation transportISS:BHF-UCL
Cellular response to acidic pHISS:BHF-UCL
Detection of chemical stimulus involved in sensory perception of sour tasteISS:BHF-UCL
Detection of chemical stimulus involved in sensory perception of tasteISS:UniProtKB
Detection of mechanical stimulusManual Assertion Based On ExperimentIBA:GO_Central
Inorganic cation transmembrane transportManual Assertion Based On ExperimentIDA:UniProtKB
Potassium ion transmembrane transportManual Assertion Based On ExperimentIDA:BHF-UCL
Protein homotetramerizationManual Assertion Based On ExperimentIDA:UniProtKB
Response to waterIEA:Ensembl
Sensory perception of sour tasteManual Assertion Based On ExperimentIMP:UniProtKB
Smoothened signaling pathwayISS:UniProtKB
Sodium ion transmembrane transportManual Assertion Based On ExperimentIDA:BHF-UCL

Cellular Location

Cell projection, cilium membrane
Cell membrane
Cytoplasmic vesicle
Interaction with PKD1 or PKD1L3 is required for localization to the cell membrane.

Topology

Cytoplasmic: 1-103
Helical: 104-124
Extracellular: 125-356
Helical: 357-376
Cytoplasmic: 377-384
Helical: 385-405
Extracellular: 406-433
Helical: 434-454
Cytoplasmic: 455-479
Helical: 480-499
Extracellular: 500-511
Helical: 512-526
Cytoplasmic: 527-536
Helical: 537-557
Extracellular: 558-805

PTM

Palmitoylation is important for expression at the cell membrane and for channel activity.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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