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Mouse Anti-PLOD3 Recombinant Antibody (CBYC-P450) (CBMAB-P2160-YC)

Provided herein is a Mouse monoclonal antibody against Human Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 3. The antibody can be used for immunoassay techniques, such as ELISA, WB, IHC, IF, IP.
See all PLOD3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYC-P450
Antibody Isotype
IgG1
Application
ELISA, WB, IHC, IF, IP

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 3
Introduction
PLOD3 is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks.
Entrez Gene ID
8985
UniProt ID
O60568
Alternative Names
Lysyl Hydroxlase 3; LH3
Function
Multifunctional enzyme that catalyzes a series of essential post-translational modifications on Lys residues in procollagen (PubMed:11956192, PubMed:12475640, PubMed:18298658, PubMed:30089812, PubMed:18834968).
Plays a redundant role in catalyzing the formation of hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (PubMed:9582318, PubMed:9724729, PubMed:11956192, PubMed:12475640, PubMed:18298658, PubMed:30089812, PubMed:18834968).
Plays a redundant role in catalyzing the transfer of galactose onto hydroxylysine groups, giving rise to galactosyl 5-hydroxylysine (PubMed:12475640, PubMed:18298658, PubMed:30089812, PubMed:18834968).
Has an essential role by catalyzing the subsequent transfer of glucose moieties, giving rise to 1,2-glucosylgalactosyl-5-hydroxylysine residues (PubMed:10934207, PubMed:11896059, PubMed:11956192, PubMed:12475640, PubMed:18298658, PubMed:30089812, PubMed:18834968).
Catalyzes hydroxylation and glycosylation of Lys residues in the MBL1 collagen-like domain, giving rise to hydroxylysine and 1,2-glucosylgalactosyl-5-hydroxylysine residues (PubMed:25419660).
Essential for normal biosynthesis and secretion of type IV collagens (PubMed:18834968) (Probable). Essential for normal formation of basement membranes (By similarity).
Biological Process
Basement membrane assemblyIEA:Ensembl
Collagen fibril organizationTAS:Reactome
Collagen metabolic processIEA:Ensembl
Endothelial cell morphogenesisIEA:Ensembl
Epidermis morphogenesisIEA:Ensembl
Hydroxylysine biosynthetic processIEA:Ensembl
In utero embryonic developmentIEA:Ensembl
Lung morphogenesisIEA:Ensembl
Neural tube developmentIEA:Ensembl
Peptidyl-lysine hydroxylationManual Assertion Based On ExperimentIDA:CAFA
Protein localizationIEA:Ensembl
Protein O-linked glycosylationManual Assertion Based On ExperimentIMP:CAFA
VasodilationIEA:Ensembl
Cellular Location
Rough endoplasmic reticulum
Endoplasmic reticulum lumen
Endoplasmic reticulum membrane
Secreted
Secreted, extracellular space
The majority of the secreted protein is associated with the extracellular matrix.
Involvement in disease
Lysyl hydroxylase 3 deficiency (LH3 deficiency):
Connective tissue disorder. The syndrome is characterized by congenital malformations severely affecting many tissues and organs and revealing features of several collagen disorders, most of them involving COL2A1 (type II collagen). The findings suggest that the failure of lysyl hydroxylation and hydroxylysyl carbohydrate addition, which affects many collagens, is the molecular basis of this syndrome.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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