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Mouse Anti-PLSCR1 Recombinant Antibody (3B4) (CBMAB-P2178-YC)

Provided herein is a Mouse monoclonal antibody against Human Phospholipid Scramblase 1. The antibody can be used for immunoassay techniques, such as ELISA, IP.
See all PLSCR1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3B4
Antibody Isotype
IgG2a, κ
Application
ELISA, IP

Basic Information

Immunogen
PLSCR1 (AAH32718, 1-318 aa) full length recombinant protein with GST tag. Immunogen sequence: MDKQNSQMNA SHPETNLPVG YPPQYPPTAF QGPPGYSGYP GPQVSYPPPP AGHSGPGPAG FPVPNQPVYN QPVYNQPVGA AGVPWMPAPQ PPLNCPPGLE YLSQIDQILI HQQIKLLEVL TGFETNNKYE IKNSFGQRVY FAAEDTDCCT RNCCGPSRPF TLRIIDNMGQ EVITLERPLR CSSCCCPCCL QEIEIQAPPG VPIGYVIQTW HPCLPKFTIQ NEKREDVLKI SGPCVVCSCC GDVDFEIKSL DEQCVVGKIS KHWTGILREA FTDADNFGIQ FPLDLDVKMK AVMIGACFLI DFMFFESTGS QEQKSGVW
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-318

Target

Full Name
PLSCR1
Introduction
PLSCR1 (Phospholipid Scramblase 1) is a protein coding gene. Diseases associated with PLSCR1 include Scott Syndrome. Among its related pathways are EGF/EGFR Signaling Pathway. Gene Ontology annotations related to this gene include calcium ion binding and transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of the gene is PLSCR2.
Entrez Gene ID
5359
UniProt ID
O15162
Alternative Names
MMTRA1B
Function
Catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer leaflet of the plasma membrane resulting in collapse of the phospholipid asymmetry which leads to phosphatidylserine externalization on the cell surface (PubMed:9218461, PubMed:8663431, PubMed:10770950, PubMed:9572851, PubMed:9485382, PubMed:18629440, PubMed:23590222, PubMed:24648509, PubMed:24343571, PubMed:32110987, PubMed:23659204, PubMed:29748552).
Mediates calcium-dependent phosphatidylserine externalization and apoptosis in neurons via its association with TRPC5 (By similarity).
Also exhibits magnesium-dependent nuclease activity against double-stranded DNA and RNA but not single-stranded DNA and can enhance DNA decatenation mediated by TOP2A (PubMed:27206388, PubMed:17567603).
Negatively regulates FcR-mediated phagocytosis in differentiated macrophages (PubMed:26745724).
May contribute to cytokine-regulated cell proliferation and differentiation (By similarity).
May play a role in the antiviral response of interferon (IFN) by amplifying and enhancing the IFN response through increased expression of select subset of potent antiviral genes (PubMed:15308695).
Acts as an attachment receptor for HCV (PubMed:21806988).
Biological Process
Acute-phase responseISS:UniProtKB
Apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Defense response to virusManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of phagocytosisManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of viral genome replicationManual Assertion Based On ExperimentIMP:UniProtKB
Phosphatidylserine biosynthetic processISS:UniProtKB
Phosphatidylserine exposure on apoptotic cell surfaceManual Assertion Based On ExperimentIMP:UniProtKB
Plasma membrane phospholipid scramblingManual Assertion Based On ExperimentIDA:UniProtKB
Platelet activation1 PublicationNAS:UniProtKB
Positive regulation of chromosome separationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of DNA topoisomerase (ATP-hydrolyzing) activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of gene expressionManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of innate immune responseManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of Fc receptor mediated stimulatory signaling pathwayISS:UniProtKB
Regulation of mast cell activationISS:UniProtKB
Response to interferon-betaManual Assertion Based On ExperimentIMP:UniProtKB
Response to lead ionManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cell membrane
Nucleus
Cytoplasm
Cytoplasm, perinuclear region
Localizes to the perinuclear region in the presence of RELT (PubMed:22052202).
Palmitoylation regulates its localization to the cell membrane or the nucleus; trafficking to the cell membrane is dependent upon palmitoylation whereas in the absence of palmitoylation, localizes to the nucleus (PubMed:12564925).
Topology
Cytoplasmic: 1-288
Helical: 289-305
Extracellular: 306-318
PTM
Phosphorylation at Thr-161 by PKC/PKCD increases its phospholipid scramblase activity during both cell stimulation and apoptosis (PubMed:10770950).
Phosphorylated by OXSR1 in the presence of RELT
Palmitoylation is required for its phospholipid scramblase activity (PubMed:9572851).
Palmitoylation regulates its localization to the cell membrane or the nucleus; trafficking to the cell membrane is dependent upon palmitoylation whereas in the absence of palmitoylation, localizes to the nucleus (PubMed:12564925).
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For research use only. Not intended for any clinical use.

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