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Mouse Anti-PMEL Recombinant Antibody (CBT2732) (V2LY-0625-LY1814)


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Tested Data
Mouse Anti-PMEL Recombinant Antibody (CBT2732) (V2LY-0625-LY1814) in ELISA
Mouse Anti-PMEL Recombinant Antibody (CBT2732) (V2LY-0625-LY1814) in ELISA
Mouse Anti-PMEL Recombinant Antibody (CBT2732) (V2LY-0625-LY1814) in WB
Mouse Anti-PMEL Recombinant Antibody (CBT2732) (V2LY-0625-LY1814) in WB
Mouse Anti-PMEL Recombinant Antibody (CBT2732) (V2LY-0625-LY1814) in WB
Mouse Anti-PMEL Recombinant Antibody (CBT2732) (V2LY-0625-LY1814) in WB
Mouse Anti-PMEL Recombinant Antibody (CBT2732) (V2LY-0625-LY1814) in FC
Mouse Anti-PMEL Recombinant Antibody (CBT2732) (V2LY-0625-LY1814) in FC

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT2732
Antibody Isotype
IgG1
Application
FC

Basic Information

Immunogen
Purified recombinant fragment of human PMEL (AA: 25-192) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Premelanosome protein
Entrez Gene ID
6490
UniProt ID
P40967
Function
Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
Biological Process
Melanin biosynthetic processIEA:UniProtKB-KW
Melanosome organizationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Endoplasmic reticulum membrane
Golgi apparatus
Melanosome
Endosome, multivesicular body
Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes.
M-alpha
Secreted
Topology
Lumenal: 470-595
Helical: 596-616
Cytoplasmic: 617-661
PTM
A small amount of P1/P100 (major form) undergoes glycosylation to yield P2/P120 (minor form). P2 is cleaved by a furin-like proprotein convertase (PC) in a pH-dependent manner in a post-Golgi, prelysosomal compartment into two disulfide-linked subunits: a large lumenal subunit, M-alpha/ME20-S, and an integral membrane subunit, M-beta. Despite cleavage, only a small fraction of M-alpha is secreted, whereas most M-alpha and M-beta remain associated with each other intracellularly. M-alpha is further processed to M-alpha N and M-alpha C. M-alpha C further undergoes processing to yield M-alpha C1 and M-alpha C3 (M-alpha C2 in the case of PMEL17-is or PMEL17-ls). Formation of intralumenal fibrils in the melanosomes requires the formation of M-alpha that becomes incorporated into the fibrils. Stage II melanosomes harbor only Golgi-modified Pmel17 fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides.
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For research use only. Not intended for any clinical use.

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