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Mouse Anti-POFUT1 Recombinant Antibody (3H1) (CBMAB-P2290-YC)

Provided herein is a Mouse monoclonal antibody against Human Protein O-Fucosyltransferase 1. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all POFUT1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3H1
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
POFUT1 (NP_758436, 85-194 aa) partial recombinant protein with GST tag. Immunogen sequence: VSYQKYFKLE PLQAYHRVIS LEDFMEKLAP THWPPEKRVA YCFEVAAQRS PDKKTCPMKE GNPFGPFWDQ FHVSFNKSEL FTGISFSASY REQWSQRREN HSCVTLLFPR
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 85-194

Target

Full Name
protein O-fucosyltransferase 1
Introduction
POFUT1 (Protein O-Fucosyltransferase 1) is a protein coding gene. Diseases associated with POFUT1 include Dowling-Degos Disease 2 and Dowling-Degos Disease. Among its related pathways are Notch Signaling Pathway (sino) and Pre-NOTCH Expression and Processing. Gene Ontology annotations related to this gene include fucosyltransferase activity and peptide-O-fucosyltransferase activity.
Entrez Gene ID
23509
UniProt ID
Q9H488
Alternative Names
FUT12; DDD2; O-FUT; OFUCT1; O-FucT-1; O-Fuc-T
Function
Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs).
Biological Process
AngiogenesisIEA:Ensembl
Fucose metabolic processIEA:UniProtKB-KW
Heart developmentIEA:Ensembl
Nervous system developmentIEA:Ensembl
Notch signaling pathway1 PublicationNAS:UniProtKB
O-glycan processingManual Assertion Based On ExperimentTAS:UniProtKB
Protein O-linked fucosylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein O-linked glycosylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of Notch signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of transcription, DNA-templated1 PublicationNAS:UniProtKB
SomitogenesisIEA:Ensembl
Cellular Location
Endoplasmic reticulum
Involvement in disease
Dowling-Degos disease 2 (DDD2):
An autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails.
PTM
N-glycosylated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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