Mouse Anti-POLK Recombinant Antibody (6F2) (CBMAB-D2188-YC)

Name: Mouse Anti-POLK Recombinant Antibody (6F2)
SKU: CBMAB-D2188-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

6F2

Application

ELISA, ICC, IF, IHC-P, WB

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

POLK

Introduction

POLK is a member of the DNA polymerase type-Y family of proteins. POLK is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Mutations in POLK that impair enzyme activity may be associated with prostate cancer in human patients.

Entrez Gene ID

51426

UniProt ID

Q9UBT6

Alternative Names

DINP; POLQ; DINB1

Function

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.

Biological Process

Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to UVManual Assertion Based On ExperimentIDA:UniProtKB
DNA repairManual Assertion Based On ExperimentIDA:UniProtKB
DNA replicationIEA:UniProtKB-KW
Error-prone translesion synthesisManual Assertion Based On ExperimentIDA:UniProtKB
Nucleotide-excision repair, DNA gap fillingManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Nucleus
Detected throughout the nucleus and at replication foci (PubMed:12414988).
Recruited to DNA damage sites in response to ultraviolet irradiation: N6-methyladenosine (m6A)-containing mRNAs accumulate in the vicinity of DNA damage sites and their presence is required to recruit POLK (PubMed:28297716).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

POLK Matched Antibody Pair (906) (CAT#: APMAB-906LY)

Related Products

Mouse Anti-POLK Recombinant Antibody (CBYC-P499) (CAT#: CBMAB-P2335-YC)

Mouse Anti-POLK Recombinant Antibody (4F12) (CAT#: CBMAB-P2333-YC)

POLK Matched Antibody Pair (906) (CAT#: APMAB-906LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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