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Mouse Anti-POLK Recombinant Antibody (6F2) (CBMAB-D2188-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DNA Polymerase Kappa (POLK). The antibody can be used for immunoassay techniques, such as ELISA, ICC, IF, IHC-P, WB.
See all POLK antibodies
Published Data
Fig.1 The relative expression of hpol κ was examined by immunoblotting using total cell lysate from three glioblastoma-derived cell lines (T98G, U-87-MG, and A172) as well as NHAs.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
6F2
Antibody Isotype
IgG1
Application
ELISA, ICC, IF, IHC-P, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
POLK
Introduction
POLK is a member of the DNA polymerase type-Y family of proteins. POLK is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Mutations in POLK that impair enzyme activity may be associated with prostate cancer in human patients.
Entrez Gene ID
51426
UniProt ID
Q9UBT6
Alternative Names
DINP; POLQ; DINB1
Function
DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
Biological Process
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to UVManual Assertion Based On ExperimentIDA:UniProtKB
DNA repairManual Assertion Based On ExperimentIDA:UniProtKB
DNA replicationIEA:UniProtKB-KW
Error-prone translesion synthesisManual Assertion Based On ExperimentIDA:UniProtKB
Nucleotide-excision repair, DNA gap fillingManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Nucleus
Detected throughout the nucleus and at replication foci (PubMed:12414988).
Recruited to DNA damage sites in response to ultraviolet irradiation: N6-methyladenosine (m6A)-containing mRNAs accumulate in the vicinity of DNA damage sites and their presence is required to recruit POLK (PubMed:28297716).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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