Mouse Anti-POLR2D Recombinant Antibody (1E4-A5) (CBMAB-R0643-CN)

Name: Mouse Anti-POLR2D Recombinant Antibody (1E4-A5)
SKU: CBMAB-R0643-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

1E4-A5

Application

ELISA, WB

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.2

More Infomation

Target

Full Name

polymerase (RNA) II (DNA directed) polypeptide D

Introduction

This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq, Jul 2008]

Entrez Gene ID

5433

UniProt ID

O15514

Alternative Names

RBP4; RPB16; HSRBP4; HSRPB4

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity).

Biological Process

mRNA export from nucleus in response to heat stressManual Assertion Based On ExperimentIBA:GO_Central
Nuclear-transcribed mRNA catabolic process, deadenylation-dependent decayManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of translational initiationManual Assertion Based On ExperimentIBA:GO_Central
Recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complexManual Assertion Based On ExperimentIBA:GO_Central
Transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Transcription initiation from RNA polymerase II promoterManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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