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Mouse Anti-POLR2E Recombinant Antibody (3B5) (CBMAB-P2353-YC)

Provided herein is a Mouse monoclonal antibody against Human RNA Polymerase II Subunit E. The antibody can be used for immunoassay techniques, such as WB, IHC, IF, FC.
See all POLR2E antibodies
Published Data
Fig.1 Western blot analysis of POLR2E expression levels in irradiated cells.
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Fig.1 Western blot analysis with anti-POLR2E antibody.
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Summary

Host Animal
Mouse
Specificity
Human, Dog, Rat, Monkey, Mouse
Clone
3B5
Antibody Isotype
IgG2a
Application
WB, IHC, IF, FC

Basic Information

Specificity
Human, Dog, Rat, Monkey, Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
RNA Polymerase II Subunit E
Introduction
POLR2E is the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.
Entrez Gene ID
Human5434
Mouse66420
Rat690966
Monkey721178
Dog611859
UniProt ID
HumanP19388
MouseQ80UW8
RatB0BNE2
MonkeyI2CYA3
DogF6X571
Alternative Names
RPABC1; RPB5; XAP4; hRPB25; hsRPB5
Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity).
Biological Process
Protein stabilization1 PublicationIC:ComplexPortal
Transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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