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Mouse Anti-POLR3K Recombinant Antibody (3F5) (CBMAB-P2379-YC)

Provided herein is a Mouse monoclonal antibody against Human RNA Polymerase III Subunit K. The antibody can be used for immunoassay techniques, such as WB.
See all POLR3K antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3F5
Antibody Isotype
IgG1, κ
Application
WB

Basic Information

Immunogen
POLR3K (AAH11932, 1-109 aa) full length recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
POLR3K
Introduction
POLR3K is a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription.
Entrez Gene ID
51728
UniProt ID
Q9Y2Y1
Alternative Names
RPC12.5; RPC11; RPC10; C11-RNP3; My010; C11
Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed:12391170).
Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs (PubMed:12391170, PubMed:30584594).
Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses (PubMed:19631370, PubMed:19609254).
Acts as nuclear and cytosolic DNA sensor involved in innate immune response (PubMed:19631370, PubMed:19609254).
Can sense non-self dsDNA that serves as template for transcription into dsRNA (PubMed:19631370, PubMed:19609254).
The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed:19631370, PubMed:19609254).
Biological Process
Defense response to virusIEA:UniProtKB-KW
Innate immune responseIEA:UniProtKB-KW
Termination of RNA polymerase III transcriptionManual Assertion Based On ExperimentIBA:GO_Central
Transcription by RNA polymerase IIIManual Assertion Based On ExperimentTAS:ProtInc
tRNA 3'-trailer cleavageIEA:InterPro
Cellular Location
Nucleus, nucleolus
Involvement in disease
Leukodystrophy, hypomyelinating, 21 (HLD21):
An autosomal recessive neurodegenerative disorder characterized by global developmental delay, loss of motor, speech and cognitive milestones in the first decades of life, and diffuse hypomyelination of the white matter and atrophy of the cerebellum and corpus callosum observed on brain imaging. Clinical features include nystagmus, ataxia, dystonia, and spasticity. Other more variable features are feeding difficulties, poor overall growth with microcephaly, optic atrophy, and seizures.The disorder is progressive and may lead to premature death.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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