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Mouse Anti-PPHLN1 Recombinant Antibody (4B6) (CBMAB-P2508-YC)

Provided herein is a Mouse monoclonal antibody against Human Periphilin 1. The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all PPHLN1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4B6
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
PPHLN1 (NP_958846.1, 168-258 aa) partial recombinant protein with GST tag. Immunogen sequence: KVLDKPSRLT EKELAEAASK WAAEKLEKSD ESNLPEISEY EAGSTAPLFT DQPEEPESNT THGIELFEDS QLTTRSKAIA SKTKEIEQVR V
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 168-258

Target

Full Name
periphilin 1
Introduction
PPHLN1 is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation.
Entrez Gene ID
51535
UniProt ID
Q8NEY8
Alternative Names
CR; HSPC232; HSPC206
Function
Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. In the HUSH complex, contributes to the maintenance of the complex at chromatin (PubMed:26022416).
Acts as a transcriptional corepressor and regulates the cell cycle, probably via the HUSH complex (PubMed:15474462, PubMed:17963697).
The HUSH complex is also involved in the silencing of unintegrated retroviral DNA: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed:30487602).
May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation (PubMed:12853457).
Biological Process
KeratinizationIEA:UniProtKB-KW
Negative regulation of gene expression, epigeneticManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription, DNA-templatedIEA:InterPro
Positive regulation of DNA methylation-dependent heterochromatin assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Protein localization to heterochromatinManual Assertion Based On ExperimentIDA:UniProtKB
PTM
Substrate of transglutaminase (in vitro).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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