Mouse Anti-PPHLN1 Recombinant Antibody (4B6) (CBMAB-P2508-YC)

Name: Mouse Anti-PPHLN1 Recombinant Antibody (4B6)
SKU: CBMAB-P2508-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

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Basic Information

Host Animal

Mouse

Clone

4B6

Application

ELISA, IF, WB

Immunogen

PPHLN1 (NP_958846.1, 168-258 aa) partial recombinant protein with GST tag. Immunogen sequence: KVLDKPSRLT EKELAEAASK WAAEKLEKSD ESNLPEISEY EAGSTAPLFT DQPEEPESNT THGIELFEDS QLTTRSKAIA SKTKEIEQVR V

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 168-258

More Infomation

Target

Full Name

periphilin 1

Introduction

PPHLN1 is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation.

Entrez Gene ID

51535

UniProt ID

Q8NEY8

Alternative Names

CR; HSPC232; HSPC206

Function

Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. In the HUSH complex, contributes to the maintenance of the complex at chromatin (PubMed:26022416).
Acts as a transcriptional corepressor and regulates the cell cycle, probably via the HUSH complex (PubMed:15474462, PubMed:17963697).
The HUSH complex is also involved in the silencing of unintegrated retroviral DNA: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed:30487602).
May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation (PubMed:12853457).

Biological Process

KeratinizationIEA:UniProtKB-KW
Negative regulation of gene expression, epigeneticManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription, DNA-templatedIEA:InterPro
Positive regulation of DNA methylation-dependent heterochromatin assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Protein localization to heterochromatinManual Assertion Based On ExperimentIDA:UniProtKB

PTM

Substrate of transglutaminase (in vitro).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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