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Rabbit Anti-PPP1R12A Recombinant Antibody (D6C1) (CBMAB-CP1635-LY)

The product is antibody recognizes PPP1R12A. The antibody D6C1 immunoassay techniques such as: WB,IP,IF (ICC).
See all PPP1R12A antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Monkey, Dog
Clone
D6C1
Antibody Isotype
IgG
Application
WB, IP, IF (ICC)

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MYPT1 protein.
Specificity
Human, Monkey, Dog
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Protein Phosphatase 1 Regulatory Subunit 12A
Entrez Gene ID
Human4659
Monkey695735
Dog475411
UniProt ID
HumanO14974
MonkeyI0FRY0
DogJ9P5T7
Function
Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.
Biological Process
Cellular response to xenobiotic stimulusIEA:Ensembl
Centrosome cycleManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of catalytic activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of myosin-light-chain-phosphatase activityManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Protein dephosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of cell adhesionManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of myosin-light-chain-phosphatase activityManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of nucleocytoplasmic transportIEA:Ensembl
Signal transductionIEA:InterPro
Cellular Location
Cytoplasm
Cytoplasm, cytoskeleton, stress fiber
Also along actomyosin filaments.
Involvement in disease
Genitourinary and/or brain malformation syndrome (GUBS):
An autosomal dominant syndrome characterized by multiple congenital anomalies including urogenital malformations and brain abnormalities ranging from agenesis of the corpus callosum to anencephaly.
PTM
Phosphorylated by CIT (Rho-associated kinase) (By similarity).
Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK; may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates PPP1R12A.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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