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Mouse Anti-PRAME Recombinant Antibody (2B4) (CBMAB-P2682-YC)

Provided herein is a Mouse monoclonal antibody against Human Preferentially Expressed Antigen In Melanoma. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all PRAME antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2B4
Antibody Isotype
IgG
Application
ELISA, WB

Basic Information

Immunogen
PRAP1 (NP_660203.2, aa1-151) full length recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Preferentially Expressed Antigen In Melanoma
Introduction
PRAME is an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function.
Entrez Gene ID
23532
UniProt ID
P78395
Alternative Names
OIP-4; OIP4; CT130; MAPE
Function
Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation (PubMed:21822215, PubMed:26138980).
The CRL2(PRAME) complex mediates ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec decoding (PubMed:26138980).
In the nucleus, the CRL2(PRAME) complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY) and probably plays a role in chromstin regulation (PubMed:21822215).
Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG: prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis (PubMed:16179254).
Biological Process
Apoptotic processIEA:UniProtKB-KW
Cell differentiationIEA:UniProtKB-KW
Negative regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of cell differentiationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of retinoic acid receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of cell population proliferationManual Assertion Based On ExperimentIDA:UniProtKB
Proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Protein polyubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of growthIEA:UniProtKB-KW
Cellular Location
Nucleus
Chromosome
Cytoplasm
Golgi apparatus
Cell membrane
Associates with chromatin; specifically enriched at transcriptionally active promoters that are also bound by nuclear transcription factor Y (composed of NFYA, NFYB and NFYC) and at enhancers (PubMed:21822215).
Recruited to the Golgi apparatus in response to interferon gamma (IFNG) treatment (PubMed:23460923).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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